Effect of G-CSF on peripheral blood progenitor cell mobilization and collection from healthy donors.

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dc.contributor.authorChaisiripoomkere, Wen_US
dc.contributor.authorJootar, Sen_US
dc.contributor.authorUngkanont, Aen_US
dc.date.accessioned2009-05-27T17:20:38Z
dc.date.available2009-05-27T17:20:38Z
dc.date.issued2001-09-06en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractWe studied granulocyte colony-stimulating factor (G-CSF) mediated peripheral blood progenitor cells (PBPC), which were mobilized and collected from healthy donors for allogeneic transplantation. A total of 26 donors, age ranged from 21-41 years were mobilized with G-CSF at a dose of 7.5 microg/kg/day subcutaneously for 5 days and the collection was started on day 5. The CD34 cell counts reached a maximum on day 5 and subsequently declined despite continually given G-CSF. White blood cells (WBC), absolute neutrophil counts (ANC), absolute lymphocytes (AL) and their subsets, absolute mononuclear cells (AMNC), colony-forming unit-granulocyte, macrophage (CFU-GM) and CD34+ cells were increased about 6, 9, 2, 3, 34 and 40-fold, respectively, but red blood cells (RBC), hematocrit (Hct) and platelets (Pit) decreased on day 5 when compared to day 0. All parameters decreased after stem cell collection. For stem cell collection by Cobe Spectra, we used a blood volume of 19.27 +/- 4.65 liters, flow rate of 60.53 +/- 10.03 ml/minute, acid citrate dextrose solution (ACD)/blood ratio of 1:13.31, the final product volume was 314.14 +/- 72.24 ml, collection time was 325.40 +/- 73.36 minutes and one or two procedures were sufficient. The correlation between the number of CD34+ cells/kg, CFU-GM/kg and MNC/kg found in the harvested product and CD34 cells can be used for determining the necessary amount of progenitor cells for transplantation.en_US
dc.description.affiliationResearch Center Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationChaisiripoomkere W, Jootar S, Ungkanont A. Effect of G-CSF on peripheral blood progenitor cell mobilization and collection from healthy donors. Asian Pacific Journal of Allergy and Immunology. 2001 Sep; 19(3): 183-90en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36985
dc.language.isoengen_US
dc.subject.meshAdulten_US
dc.subject.meshAntigens, CD34 --blooden_US
dc.subject.meshBlood Cell Counten_US
dc.subject.meshColony-Forming Units Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshFilgrastim --pharmacologyen_US
dc.subject.meshGranulocyte Colony-Stimulating Factor --pharmacologyen_US
dc.subject.meshHematopoietic Stem Cell Mobilization --methodsen_US
dc.subject.meshHematopoietic Stem Cell Transplantationen_US
dc.subject.meshHematopoietic Stem Cells --cytologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukapheresisen_US
dc.subject.meshLeukocytes, Mononuclear --cytologyen_US
dc.subject.meshMaleen_US
dc.subject.meshRecombinant Proteins --pharmacologyen_US
dc.subject.meshTransplantation, Homologousen_US
dc.titleEffect of G-CSF on peripheral blood progenitor cell mobilization and collection from healthy donors.en_US
dc.typeJournal Articleen_US
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