MicroFLOQ Direct: A Helpful Tool for the Coronavirus SARS-Cov-2 Rapid Detection without RNA Purification

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dc.contributor.authorRecipon, Mathildeen_US
dc.contributor.authorPussiau, Amauryen_US
dc.contributor.authorFollot, Sébastienen_US
dc.contributor.authorLatifa, Noussairen_US
dc.contributor.authorHerrmann, Jean-Louisen_US
dc.contributor.authorRottman, Martinen_US
dc.contributor.authorGallet, Olivieren_US
dc.contributor.authorKellouche, Sabrinaen_US
dc.contributor.authorLeroy-Dudal, Johanneen_US
dc.contributor.authorPierrini, Georgesen_US
dc.contributor.authorMarescal, Francken_US
dc.contributor.authorTouron, Patricken_US
dc.contributor.authorHubac, Sylvainen_US
dc.contributor.authorSiatka, Christianen_US
dc.date.accessioned2023-08-25T06:43:53Z
dc.date.available2023-08-25T06:43:53Z
dc.date.issued2022-03
dc.description.abstractIn the context of SARS-Cov-2 virus disease (Covid19) pandemic, molecular diagnostic tools were rapidly developed as there are fundamental for a rapid detection of infected people. In this context, and in order to optimize the manipulations and reduce the time to get results, we report the successful use of a sampling tool for Covid19 diagnosis named microFLOQ® Direct (MFD). Hundred upper respiratory specimens sampled from patients with potential Covid19 were evaluated using MFD, and results were compared to the results obtained by standard sampling procedure using dry swabs and physiologic serum as the transport medium. MFD results compared to results issued from the classic RNA purification and amplification steps from transport medium showed that MFD can be directly used for RT-PCR analysis without the preliminary inactivation and extraction steps. So, MFD could limit handling errors compared to the different treatment steps with dry swabs and transport medium, and therefore the risk of operator contamination, and could simplify the analytical process and enables to get results in less than 2 hours. We expect that the proposed detection kit using MFD sampling represents a relevant and operational screening tool in the field of molecular detection of viral and bacterial diseases during pandemic or for public health or agro-veterinary purposes.en_US
dc.identifier.affiliationsInstitut de Recherche Criminelle de la Gendarmerie Nationale (IRCGN, 5 Boulevard de l'Hautil, 95000 Cergy – Franceen_US
dc.identifier.affiliationsCY Cergy Paris University, Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellule (ERRMECE, Mec’uP group, rue Descartes 95000 Neuville-sur-Oise - Franceen_US
dc.identifier.affiliationsAP-HP, GHU Paris-Saclay, Hôpital Raymond Poincaré, Garches, Franceen_US
dc.identifier.affiliationsUniversité Paris-Saclay, UVSQ, INSERM, Infection et Inflammation (U1173), 78000 Montigny le Bretonneux, Franceen_US
dc.identifier.affiliationsGroupement de gendarmerie départementale Quartier Gendarme Dupré, 43 rue du Maréchal Leclerc, 28110 Luce Franceen_US
dc.identifier.affiliationsPôle Judiciaire de la Gendarmerie Nationale (PJGN), 5 Boulevard de l'Hautil, 95000 Cergy – Franceen_US
dc.identifier.affiliationsNîmes University, UPR CHROME, 7 Place Gabriel Péri, 30000 Nîmes – Franceen_US
dc.identifier.affiliationsEcole de l’ADN, 19 grand rue, Muséum d’Histoire Naturelle, 30000 Nîmes – Franceen_US
dc.identifier.citationRecipon Mathilde, Pussiau Amaury, Follot Sébastien, Latifa Noussair, Herrmann Jean-Louis, Rottman Martin, Gallet Olivier, Kellouche Sabrina, Leroy-Dudal Johanne, Pierrini Georges, Marescal Franck, Touron Patrick, Hubac Sylvain, Siatka Christian. MicroFLOQ Direct: A Helpful Tool for the Coronavirus SARS-Cov-2 Rapid Detection without RNA Purification. International Journal of Applied Biology and Pharmaceutical Technology. 2022 Mar; 13(1): 1-13en_US
dc.identifier.issn0976-4550
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/225562
dc.languageenen_US
dc.publisherApplied Biology and Pharmaceutical Sciencesen_US
dc.relation.issuenumber1en_US
dc.relation.volume13en_US
dc.source.urihttps://www.doi.org/10.26502/ijabpt.202115en_US
dc.subjectmicroFLOQ®en_US
dc.subjectSARS-Cov-2en_US
dc.subjectRT-PCRen_US
dc.titleMicroFLOQ Direct: A Helpful Tool for the Coronavirus SARS-Cov-2 Rapid Detection without RNA Purificationen_US
dc.typeJournal Articleen_US
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