Investigation of chromosomal aberrations in Egyptian hepatocellular carcinoma patients by fluorescence in situ hybridization.

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dc.contributor.authorAly, Magdy S
dc.contributor.authorBahnassy, Abeer A
dc.contributor.authorAbdel-Rahman, Zekri N
dc.date.accessioned2012-06-08T09:51:28Z
dc.date.available2012-06-08T09:51:28Z
dc.date.issued2010-05
dc.description.abstractBACKGROUND AND AIMS: Hepatocellular carcinoma (HCC) is a very common and highly malignant tumor, associated mainly with chronic viral hepatitis, cirrhosis of any cause, aflatoxin exposure and ethanol consumption. Cytogenetic analysis on HCC has been limited because of poor hepatocyte growth in vitro. Conventional cytogenetic studies have demonstrated frequent abnormalities of specific chromosomes in HCC. Molecular cytogenetic approaches have been applied only rarely in the characterization of HCC. The main aim of this study was to evaluate genetic aberrations of different chromosomes in HCC. The study included 35 patients with HCC, who have been diagnosed and treated at National Cancer Institute, Cairo University, Egypt. The clinico-pathologic features of the studied patient were collected from patient’s files. MATERIALS AND METHODS: Interphase cytogenetics by fluorescence in situ hybridization with the use of a panel of centromere-associated DNA probes for chromosomes 1, 4, 8, 9, 13, 17, 20 and Y were performed on paraffin-embedded HCC specimens. RESULTS: The most common chromosomal aberrations detected were gain of chromosomes 8 in 12 cases (34.28%), 17 in 6 cases (17.14%). Loss of chromosome Y was detected in 6 of male cases (30%). Monosomy 4 was also detected in 5 cases (14.28%). Negative correlation could be detected only between chromosome 4 and 8. (r = -0.381, P < 0.05). Correlations between gain or loss of chromosomes and the different clinicopathologic parameters in the patients investigated, indicated negative correlation between: chromosome Y and age and chromosome 1 and cirrhosis. CONCLUSION: Gains and losses of DNA found in this study probably involve oncogenes and tumor suppressor genes that play a role in the puzzle of hepatocarcinogenesis.en_US
dc.identifier.citationAly Magdy S, Bahnassy Abeer A, Abdel-Rahman Zekri N. Investigation of chromosomal aberrations in Egyptian hepatocellular carcinoma patients by fluorescence in situ hybridization. Indian Journal of Human Genetics. 2010 May; 16(2): 87-93.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/138904
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2955957/en_US
dc.subjectChromosomesen_US
dc.subjectgeneticsen_US
dc.subjecthepatocellular carcinomaen_US
dc.subjectinterphase fluorescence in situ hybridizationen_US
dc.subjectliver canceren_US
dc.subject.meshCarcinoma, Hepatocellular --diagnosis
dc.subject.meshCarcinoma, Hepatocellular --epidemiology
dc.subject.meshChromosome Aberrations --genetics
dc.subject.meshCytogenetics --methods
dc.subject.meshEgypt --epidemiology
dc.subject.meshHepatitis, Viral, Human --complications
dc.subject.meshHumans
dc.subject.meshIn Situ Hybridization, Fluorescence --methods
dc.subject.meshLiver Cirrhosis --complications
dc.subject.meshPatients
dc.titleInvestigation of chromosomal aberrations in Egyptian hepatocellular carcinoma patients by fluorescence in situ hybridization.en_US
dc.typeArticleen_US
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