Fucoxanthin enhances 5-FU chemotherapeutic efficacy in colorectal cancer cells by affecting MMP-9 invasive proteins

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dc.contributor.authorManmuan, Suwisiten_US
dc.contributor.authorManmuan, Ponwiten_US
dc.date.accessioned2020-10-16T08:55:50Z
dc.date.available2020-10-16T08:55:50Z
dc.date.issued2019-12
dc.description.abstractDrug resistance is a major inconvenience which lowers the traditional chemotherapeutic efficacy and is a highly undesirable therapeutic problem which poses particular challenges in the case of colorectal cancer. Fucoxanthin is a natural orange-carotenoid, predominantly found in edible brown algae and justifiably considered as a nutritional ingredient with the capacity to powerfully enhance concurrent drug chemotherapy. It has been well-documented that fucoxanthin has good potential for anti-cancer activity while offering a remarkable range of biological activities. Accordingly, it has gained prominence in the research field as interest grows in the molecular mechanism which is associated with cancer therapy. This study was undertaken to assess the anti-cancer activity and to explore the molecular mechanism of fucoxanthin on the inhibition of cell proliferation, cell adhesion, and cell invasion, in addition to determining the synergistic effect of drug-drug combinative treatment in colorectal cancer cells. SW-620 cells were cultivated with fucoxanthin for 24, 48, 72, and 96 hours with co-treatment by 5-FU to evaluate the synergistic potential. The cell viability of cancerous cells was determined by MTT colorimetric assay. The inhibitory effects of cell invasion and adhesion were measured in the presence of fucoxanthin with 5-FU in various concentrations to determine MMP-9 gene and protein expression after treatment of the cells by RT-PCR and ELISA assay. The results illustrated that fucoxanthin profoundly inhibited cell proliferation of SW-620 cells, accompanied by arrested growth and diminished invasive ability, which was mediated at least in part by the down-regulation of MMP-9, mRNA, and protein expression. In particular, fucoxanthin strongly attenuated the anti-proliferative effect of established 5-FU by modulating the habitual hallmark of cancerous cells. These results illustrate the capacity of fucoxanthin to eradicate cancer cells and indicate the possibility that fucoxanthin could serve as a promising natural marine product derived from seaweed. The critical data in our studies will serve as the preliminary results for further studies of marine drugs in both experimental models and well-controlled clinical trialsen_US
dc.identifier.affiliationsDivision of Pharmacology, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131, Thailand.en_US
dc.identifier.affiliationsDepartment of Intellectual Property, Ministry of Commerce, Nonthaburi 11000, Thailand.en_US
dc.identifier.citationManmuan Suwisiten_US
dc.identifier.citationManmuan Ponwit. Fucoxanthin enhances 5-FU chemotherapeutic efficacy in colorectal cancer cells by affecting MMP-9 invasive proteins. Journal of Applied Pharmaceutical Science. 2019 Dec; 2019 Dec: 007-014en_US
dc.identifier.issn2231-3354
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/210498
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber12en_US
dc.relation.volume9en_US
dc.source.urihttps://dx.doi.org//10.7324/JAPS.2019.91202en_US
dc.subjectFucoxanthinen_US
dc.subjectcancer invasionen_US
dc.subjectmetastasisen_US
dc.subjectcell adhesionen_US
dc.subjectcolorectal cancer.en_US
dc.titleFucoxanthin enhances 5-FU chemotherapeutic efficacy in colorectal cancer cells by affecting MMP-9 invasive proteinsen_US
dc.typeJournal Articleen_US
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