Evaluation of a commercial Dengue NS1 enzyme-linked immunosorbent assay for early diagnosis of dengue infection.

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dc.contributor.authorShrivastava, A
dc.contributor.authorDash, P K
dc.contributor.authorTripathi, N K
dc.contributor.authorSahni, A K
dc.contributor.authorGopalan, N
dc.contributor.authorRao, P V Lakshmana
dc.date.accessioned2012-12-11T08:37:40Z
dc.date.available2012-12-11T08:37:40Z
dc.date.issued2011-01
dc.description.abstractPurpose: Dengue is one of the most serious mosquito-borne viral infections affecting tropical and subtropical countries in the world. Since there is no immunoprophylactic or specific antiviral therapy available, timely and rapid diagnosis plays a vital role in patient management and implementation of control measures. This paper evaluates a commercially available NS1 antigen capture ELISA vis-a-vis SD bioline Dengue NS1 antigen test for early detection of dengue virus. Materials and Methods: To evaluate a commercial NS1 antigen detection kit vis-a-vis SD bioline Dengue NS1 antigen test, a total of 91 clinical samples were tested. Virological investigations with regard to dengue virus, viz. NS1 antigen capture ELISA (Panbio, Australia), SD bioline Dengue NS1 antigen test, RT-PCR and virus isolation were performed. Results: Out of 91 samples, 24 (26%) were positive by NS1 antigen capture ELISA, 15 (16%) by SD bioline Dengue NS1 antigen test and 11(12%) positive by RT-PCR analysis. The RT-PCR-positive samples were further subjected to virus isolation and resulted in three isolates. The results of the Panbio NS1 antigen capture ELISA, SD bioline Dengue NS1 antigen test, RT-PCR and virus isolation were correlated among themselves. Conclusions: The present study comprehensively established the utility of NS1 antigen ELISA in early diagnosis of dengue infection.en_US
dc.identifier.citationShrivastava A, Dash P K, Tripathi N K, Sahni A K, Gopalan N, Rao P V Lakshmana. Evaluation of a commercial Dengue NS1 enzyme-linked immunosorbent assay for early diagnosis of dengue infection. Indian Journal of Medical Microbiology. 2011 Jan-Mar; 29(1): 51-55.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/143776
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2011;volume=29;issue=1;spage=51;epage=55;aulast=Shrivastavaen_US
dc.subjectAntigen captureen_US
dc.subjectdengue virusen_US
dc.subjectELISAen_US
dc.subjectlateral flowthrough testen_US
dc.subjectnon-structural 1 proteinen_US
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshAntigens, Viral --blood
dc.subject.meshClinical Laboratory Techniques --methods
dc.subject.meshDengue --diagnosis
dc.subject.meshDengue Virus --isolation & purification
dc.subject.meshEarly Diagnosis
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methods
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshReagent Kits, Diagnostic
dc.subject.meshViral Nonstructural Proteins --blood
dc.subject.meshVirology --methods
dc.subject.meshYoung Adult
dc.titleEvaluation of a commercial Dengue NS1 enzyme-linked immunosorbent assay for early diagnosis of dengue infection.en_US
dc.typeArticleen_US
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