Identification of 38kDa Brugia malayi microfilarial protease as a vaccine candidate for lymphatic filariasis.

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dc.contributor.authorKrithika, K Nen_US
dc.contributor.authorDabir, Pankajen_US
dc.contributor.authorKulkarni, Sandeepen_US
dc.contributor.authorAnandharaman, Ven_US
dc.contributor.authorReddy, M V Ren_US
dc.date.accessioned2009-05-28T12:44:52Z
dc.date.available2009-05-28T12:44:52Z
dc.date.issued2005-09-29en_US
dc.description.abstractA FPLC purified 38kDa protease (Bm mf S-7) isolated from B. malayi microfilarial soluble antigen was identified. It showed pronounced reactivity with sera collected from 'putatively immune' asymptomatic and amicrofilaraemic individuals residing in an endemic area for bancroftian filariasis. Further the immune protective activity of Bm mf S-7 antigen was evaluated in susceptible hosts, jirds (Meriones unguiculatus) against B. malayi filarial infection. The antigen showed 89% cytotoxicity against mf and 87-89% against infective (L3) larvae in in vitro antibody dependent cellular cytotoxicity Assay (ADCC) and in situ micropore chamber methods. Bm mf S-7 immunized jirds after challenge infection showed 81.5% reduction in the adult worm burden. The present study has shown that, the 38kDa microfilarial proteases (Bm mf S-7) could stimulate a strong protective immune response against microfilariae and infective larvae in jird model to block the transmission of filariasis. Analysis of IgG subclasses against Bm mf S-7 revealed a significant increase in IgG2 and IgG3 antibodies in endemic normals. Lymphocyte proliferation to Bm mf S-7 was significantly high in endemic normal group as compared to that in clinical and microfilarial carriers. Significantly enhanced levels of IFN-gamma in the culture supernatant of PBMC of endemic normals followed by stimulation with Bm mf S-7 suggest that the cellular response in this group is skewed towards Th 1 type.en_US
dc.description.affiliationDepartment of Biochemistry & Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sewagram 442 102, India.en_US
dc.identifier.citationKrithika KN, Dabir P, Kulkarni S, Anandharaman V, Reddy MV. Identification of 38kDa Brugia malayi microfilarial protease as a vaccine candidate for lymphatic filariasis. Indian Journal of Experimental Biology. 2005 Sep; 43(9): 759-68en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58499
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Helminth --chemistryen_US
dc.subject.meshAntigens, Helminth --chemistryen_US
dc.subject.meshBrugia malayi --metabolismen_US
dc.subject.meshChromatography, Affinityen_US
dc.subject.meshChromatography, Liquiden_US
dc.subject.meshCytokines --metabolismen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshElephantiasis, Filarial --prevention & controlen_US
dc.subject.meshHumansen_US
dc.subject.meshImmune Systemen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshImmunoglobulin G --chemistryen_US
dc.subject.meshInterferon-gamma --metabolismen_US
dc.subject.meshInterleukin-10 --metabolismen_US
dc.subject.meshInterleukin-4 --metabolismen_US
dc.subject.meshLeukocytes, Mononuclear --immunologyen_US
dc.subject.meshMicrofilaria --metabolismen_US
dc.subject.meshPeptide Hydrolases --chemistryen_US
dc.subject.meshTh1 Cells --immunologyen_US
dc.subject.meshTime Factorsen_US
dc.titleIdentification of 38kDa Brugia malayi microfilarial protease as a vaccine candidate for lymphatic filariasis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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