Isolation and characterization of soluble antigens of sheep poxvirus.

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dc.contributor.authorRao, T Ven_US
dc.contributor.authorNegi, B Sen_US
dc.contributor.authorBansal, M Pen_US
dc.date.accessioned2009-05-28T13:14:24Z
dc.date.available2009-05-28T13:14:24Z
dc.date.issued1997-06-01en_US
dc.description.abstractA soluble antigen fraction of sheep poxvirus (SPV) isolated from infectious virus particles by ultracentrifugation and purified by subtractive immunoaffinity chromatography was characterized. Exclusion chromatography studies revealed 10 proteins of molecular weight (MW) 220, 168, 87.3, 71.5, 52.5, 36.7, 31.0, 23.4, 18.3 and 14.2 kDa. Nine of them were found to be precipitinogens and 5 were identified as structural components of the virus particles. SDS-PAGE analysis revealed a polypeptide profile of 10 bands with 2 prominent polypeptides of 64 and 42 kDa. Western blotting, however, detected 2 immunogenic polypeptides of MW 100 and 64 kDa. Moreover, crossed immunoelectrophoresis showed the presence of proteins of varied electrophoretic mobility and sharing of antigenic determinants among a few soluble antigens. Physico-chemical characterization further revealed that these precipitinogens can withstand ambient temperatures, but were sensitive to trypsin and ether whereas, chloroform had no effect on immunoprecipitation pattern of soluble antigens.en_US
dc.description.affiliationPoxvirus Diseases Laboratory, Indian Veterinary Research Institute Campus, Mukteswar, India.en_US
dc.identifier.citationRao TV, Negi BS, Bansal MP. Isolation and characterization of soluble antigens of sheep poxvirus. Indian Journal of Experimental Biology. 1997 Jun; 35(6): 597-602en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/59147
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Viral --isolation & purificationen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshPoxviridae --immunologyen_US
dc.subject.meshSheepen_US
dc.subject.meshSolubilityen_US
dc.titleIsolation and characterization of soluble antigens of sheep poxvirus.en_US
dc.typeJournal Articleen_US
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