Elevated Expression of Notch 2 & Notch 3 is associated with Disease Progression in Colorectal Cancer

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dc.contributor.authorSharma, Abhay Kumaren_US
dc.contributor.authorNimishaen_US
dc.contributor.authorApurvaen_US
dc.contributor.authorKumar, Arunen_US
dc.contributor.authorAli, Asgaren_US
dc.contributor.authorSaluja, Sundeep Singhen_US
dc.contributor.authorPrasad, Birendraen_US
dc.date.accessioned2023-08-25T06:43:54Z
dc.date.available2023-08-25T06:43:54Z
dc.date.issued2022-12
dc.description.abstractBackground: The potential involvement of Notch signaling pathway in cell fate decision, tumor heterogeneity and angiogenesis in solid tumors can be explored in colorectal cancer (CRC). This might further help to improve outcomes in CRC. Here, the promoter methylation of Notch receptor gene (Notch2 and Notch3) and their co-expression with its downstream transcription factor Hes1 has been analyzed. Methods: Seventy-two CRC patients were enrolled to study the role of Notch2, Notch3 and Hes1 genes in colorectal cancer. Promoter methylation and mRNA expression in tumor and adjoining normal tissue were assessed by Methylation Specific PCR and quantitative Real time PCR respectively. Statistical correlation was done by using SPSS. Results: We found that Notch2 and Notch3 were hypomethylated in 52/72 (72.22%) and 54/72 (75%) cases respectively. Hypomethylation of Notch 2 and Notch 3 showed significant association with advanced stage (p=0.001) and (p=0.003) and nodal metastasis (p=0.036) and (p=0.012) respectively. Both Notch 2 and Notch 3 showed increased mRNA expression in 49 (68.05%) and 51(70.84%) patients with a fold change of 3.37 and 5.43 respectively. Positive correlation between hypomethylation and expression was observed for both genes. High expression of Hes1 was found in 53(73.61%) of cases which was highly relatable with over expression of notch receptor genes. Upregulation of Notch 2, Notch 3 and Hes1 showed significant association with high grade tumors, advance stage and presence of LN metastasis, additionally Notch 3 and Hes1 showed significant association with distant metastasis. Conclusion: Hypomethylation of Notch 2 and 3 receptors is playing crucial role in regulating the expression of these genes in CRC. Overexpression of Notch 2, Notch 3 and Hes1 are associated with disease progression in CRC.en_US
dc.identifier.affiliationsDepartment of Botany/Biotechnology, Patna University, Indiaen_US
dc.identifier.affiliationsCentral Molecular Lab, Govind Ballabh Pant Institute of Postgraduate Medical Education and Research (GIPMER), New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Gastrointestinal Surgery, Govind Ballabh Pant Institute of Postgraduate Medical Education and Research (GIPMER), New Delhi, Indiaen_US
dc.identifier.citationSharma Abhay Kumar, Nimisha, Apurva, Kumar Arun, Ali Asgar, Saluja Sundeep Singh, Prasad Birendra. Elevated Expression of Notch 2 & Notch 3 is associated with Disease Progression in Colorectal Cancer. International Journal of Applied Biology and Pharmaceutical Technology. 2022 Dec; 13(4): 33-50en_US
dc.identifier.issn0976-4550
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/225567
dc.languageenen_US
dc.publisherApplied Biology and Pharmaceutical Sciencesen_US
dc.relation.issuenumber4en_US
dc.relation.volume13en_US
dc.source.urihttps://www.doi.org/10.26502/ijabpt.202118en_US
dc.subjectColorectal Canceren_US
dc.subjectHypomethylationen_US
dc.subjectmRNA Expressionen_US
dc.subjectNotch 2en_US
dc.subjectNotch 3en_US
dc.titleElevated Expression of Notch 2 & Notch 3 is associated with Disease Progression in Colorectal Canceren_US
dc.typeJournal Articleen_US
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