In vitro seed germination of economically important edible bamboo Dendrocalamus membranaceus Munro.

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dc.contributor.authorBrar, Jasmine
dc.contributor.authorAnand, Manju
dc.contributor.authorSood, Anil
dc.date.accessioned2013-07-29T08:58:55Z
dc.date.available2013-07-29T08:58:55Z
dc.date.issued2013-01
dc.description.abstractAn in vitro propagation protocol using mature seeds of D. membranaceus was successfully established. Scarcity of seeds in bamboos because of their long flowering periods and irregular seed set resulting in low viability and germination potential, motivated us to undertake the present study. The effects of sterilants, light conditions, exogenous application of plant growth regulators and temperature in overcoming germination barriers in ageing seeds of bamboo were studied. It was found that HgCl2 (0.1%) along with bleach (15%) was more effective in raising aseptic cultures. Dark conditions, high temperatures around 30 °C and soaking of seeds in GA3 solution (50 ppm) overnight stimulated high percent of seed germination with corresponding increase in shoot length (2.7±0.7 mm) and number of sprouts (2.1±0.7) per explants during culture initiation. 6-benzylaminopurine acted synergistically with kinetin to give optimum germination rate of 70±13.9% as compared to 63.13% when used individually. For prolonged maintenance of cultures, 2% sucrose was found to be suitable for promoting photomixotrophic micropropagation. Following this procedure, about 65% survival of plantlets could be achieved during hardening. Biochemically seeds consume starchy endosperm for emergence of radicle which is taken as a sign of germination as also evident from the present study. Loss of viability and vigour after a year was confirmed by Tetrazolium chloride test. Micropropagation protocol developed here will ensure regeneration of large number of plants in a relatively short time. Conclusively, in vitro propagation protocol developed in D. membranaceus using mature seeds as an explants is reported for the first time.en_US
dc.identifier.citationBrar Jasmine, Anand Manju, Sood Anil. In vitro seed germination of economically important edible bamboo Dendrocalamus membranaceus Munro. Indian Journal of Experimental Biology. 2013 Jan; 51(1): 88-96.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/147572
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/15280en_US
dc.subjectAcclimatizationen_US
dc.subjectDendrocalamus membranaceusen_US
dc.subjectGerminationen_US
dc.subjectGibberellic aciden_US
dc.subjectLow viabilityen_US
dc.subjectSeedsen_US
dc.subject.meshEndosperm --metabolism
dc.subject.meshGermination
dc.subject.meshGibberellins --metabolism
dc.subject.meshHydrogen-Ion Concentration
dc.subject.meshIn Vitro Techniques
dc.subject.meshKinetin --chemistry
dc.subject.meshLight
dc.subject.meshPhenotype
dc.subject.meshPlant Extracts --pharmacology
dc.subject.meshPlant Leaves --metabolism
dc.subject.meshPlant Physiological Phenomena
dc.subject.meshSasa --metabolism
dc.subject.meshSeeds --metabolism
dc.subject.meshTemperature
dc.subject.meshTetrazolium Salts --pharmacology
dc.subject.meshTime Factors
dc.titleIn vitro seed germination of economically important edible bamboo Dendrocalamus membranaceus Munro.en_US
dc.typeArticleen_US
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