A method for large-scale multiplication of Curculigo orchioides through bulbil formation from leaf explant in shake flask culture.

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dc.contributor.authorSuri, S Sen_US
dc.contributor.authorArora, D Ken_US
dc.contributor.authorRamawat, K Gen_US
dc.date.accessioned2009-05-28T11:35:53Z
dc.date.available2009-05-28T11:35:53Z
dc.date.issued2000-02-24en_US
dc.description.abstractAn efficient method has been developed for large-scale multiplication of Curculigo orchioides (Hypoxidaceae), an endangered medicinal plant, through direct bulbil formation from leaf explants in shake flask cultures. Leaf-segments (7 x 10 mm) were cultured in B5 liquid medium containing KNO3 (200 mgNL-1), (NH4)2SO4 (50 mgNL-1), benzyl adenosine (2.2 microM), adenine (0.11 mM), indole butyric acid (1.0 microM) and polyvinyl pyrrolidone (250 mgL-1). About 95% of explants produced maximum number of bulbils (546/flask at 6 weeks growth) in the medium. Shake flask cultures yielded 2737 bulbils/L medium whereas static cultures yielded 624 bulbils/L medium. Germination of bulbils was maximum (90.62%) on agar-gelled B5 medium containing benzyl adenosine (2.2 microM) and gibberellic acid (3.5 microM). Plantlets developed in vitro were successfully transferred to soil with a high rate of survivability (90%) and were comparable to natural population in growth and vigour.en_US
dc.description.affiliationLaboratory of Bio-Molecular Technology, Department of Botany, M L Sukhadia University, Udaipur 313 001, India.en_US
dc.identifier.citationSuri SS, Arora DK, Ramawat KG. A method for large-scale multiplication of Curculigo orchioides through bulbil formation from leaf explant in shake flask culture. Indian Journal of Experimental Biology. 2000 Feb; 38(2): 145-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56937
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAngiosperms --growth & developmenten_US
dc.subject.meshBotany --methodsen_US
dc.subject.meshGerminationen_US
dc.subject.meshPlant Leaves --growth & developmenten_US
dc.subject.meshPlants, Medicinal --growth & developmenten_US
dc.titleA method for large-scale multiplication of Curculigo orchioides through bulbil formation from leaf explant in shake flask culture.en_US
dc.typeJournal Articleen_US
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