Comparative immunocytochemistry of isolated rat & monkey pancreatic islet cell types.

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dc.contributor.authorSujatha, S Rebeccaen_US
dc.contributor.authorPulimood, Ansuen_US
dc.contributor.authorGunasekaran, Sen_US
dc.date.accessioned2004-01-05en_US
dc.date.accessioned2009-05-27T08:29:26Z
dc.date.available2004-01-05en_US
dc.date.available2009-05-27T08:29:26Z
dc.date.issued2004-01-05en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Data on comparative distribution of the islet cell types in the Indian bonnet monkeys and rats are not available. The aim of the present study was to compare the arrangement of the three islet cell types in the native and in isolated rat and Indian bonnet monkey islets by immunocytochemistry. METHODS: Rat islets isolated by chopped tissue collagenase digestion method and islets of monkey isolated by intraductal collagenase digestion method were immunostained by streptavidin-biotin peroxidase method. RESULTS: Immunocytochemical staining of the isolated islets in both the species revealed the presence of three different cell types: insulin secreting B cells, glucagon secreting A cells and somatostatin secreting D cells. The arrangement of islet cell types in the rats and monkeys was similar to that of the intact islets of the native pancreas but were arranged in a definite pattern. In rats the A and D cells were peripherally arranged around the centrally located B cells. In monkeys the B cells occupied the majority of the periphery while the A and D cells were found mostly in the centre. INTERPRETATION & CONCLUSION: The findings of the present study showed that the arrangement of cell types in the islets was not affected by the isolation procedure. The difference in the arrangement of islet cell types in the two species may reflect special functional adaptations.en_US
dc.description.affiliationDepartment of Physiology, Christian Medical College, Vellore, India.en_US
dc.identifier.citationSujatha SR, Pulimood A, Gunasekaran S. Comparative immunocytochemistry of isolated rat & monkey pancreatic islet cell types. Indian Journal of Medical Research. 2004 Jan; 119(1): 38-44en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/24630
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiotin --chemistryen_US
dc.subject.meshCollagenases --metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshHaplorhinien_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIslets of Langerhans --cytologyen_US
dc.subject.meshIslets of Langerhans Transplantationen_US
dc.subject.meshMacaca radiataen_US
dc.subject.meshMaleen_US
dc.subject.meshPeroxidase --chemistryen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshSpecies Specificityen_US
dc.subject.meshStreptavidin --chemistryen_US
dc.subject.meshTime Factorsen_US
dc.titleComparative immunocytochemistry of isolated rat & monkey pancreatic islet cell types.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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