Effect of cholesterol and 7-beta hydroxycholesterol on glutathione status and nitric oxide production in murine peritoneal macrophages.

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dc.contributor.authorBansal, M Pen_US
dc.contributor.authorShalini, Soniaen_US
dc.date.accessioned2009-05-28T15:43:15Z
dc.date.available2009-05-28T15:43:15Z
dc.date.issued2005-06-05en_US
dc.description.abstractPresent study was conducted to observe the effect of cholesterol and oxidized cholesterol (7beta-hydroxycholesterol,7beta-OH) on the nitric oxide (NO) production and the redox ratio by lipopolysaccharide-stimulated macrophages. Dose-dependent decrease in NO levels was seen with both cholesterol and 7beta-OH at different incubation intervals (6,12,18,24 hr) and concentrations (2.5,5,7.5microg/ml). On comparison, a significant decrease in the NO was observed at 24 hr interval in 7beta-OH exposed cells with all respective concentrations of cholesterol. Incubation with 7beta-OH also resulted in significant increase in levels of oxidized glutathione (GSSG) and decrease in reduced glutathione (GSH), while cholesterol showed no effect on GSSG levels. Moreover, GSH levels were lowered only at highest concentration (7.5microg/ml), and at longer incubation intervals (18,24 hr) with cholesterol exposure. This altered the redox status in both cholesterol/7beta-OH treated macrophages. Increased redox ratio and decreased NO levels indicated increased oxidative stress and decreased vasodilation by 7beta-OH compared to cholesterol.en_US
dc.description.affiliationDepartment of Biophysics, Panjab University, Chandigarh 160 014, India. mpbansal@pu.ac.inen_US
dc.identifier.citationBansal MP, Shalini S. Effect of cholesterol and 7-beta hydroxycholesterol on glutathione status and nitric oxide production in murine peritoneal macrophages. Indian Journal of Experimental Biology. 2005 Jun; 43(6): 503-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/62329
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCholesterol --chemistryen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshFemaleen_US
dc.subject.meshGlutathione --chemistryen_US
dc.subject.meshHydroxycholesterols --chemistryen_US
dc.subject.meshLipopolysaccharides --chemistryen_US
dc.subject.meshMacrophages, Peritoneal --cytologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshNitric Oxide --chemistryen_US
dc.subject.meshNitric Oxide Synthase --metabolismen_US
dc.subject.meshOxidation-Reductionen_US
dc.subject.meshOxidative Stressen_US
dc.subject.meshOxygen --chemistryen_US
dc.subject.meshTime Factorsen_US
dc.titleEffect of cholesterol and 7-beta hydroxycholesterol on glutathione status and nitric oxide production in murine peritoneal macrophages.en_US
dc.typeJournal Articleen_US
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