Role of PGL-I of M. leprae in TNF-alpha production by in vitro whole blood assay.
| dc.contributor.author | Dhungel, S | en_US |
| dc.contributor.author | Ranjit, C | en_US |
| dc.contributor.author | Sapkota, B R | en_US |
| dc.contributor.author | Macdonald, M | en_US |
| dc.date.accessioned | 2009-05-28T04:23:19Z | |
| dc.date.available | 2009-05-28T04:23:19Z | |
| dc.date.issued | 2008-03-15 | en_US |
| dc.description | Nepal Medical College Journal. | en_US |
| dc.description.abstract | Phenolic glycolipid-I (PGL-I) is known to be a major antigen of Mycobacterium leprae. We have studied the influence of PGL-I on the production of Tumour Necrosis Factor alpha (TNF-alpha) using the in vitro whole blood assay. Armadillo-derived M. leprae (ADML) are thought to be depleted of PGL-I during the purification process. M. leprae obtained from mouse foot pad material (MFPML) has been subjected to a less rigorous purification process; their PGL-I coating is therefore believed to be more intact than that of ADML. PGL-I or ADML alone induced the secretion of minimal levels of TNF-alpha in whole blood assay; when added in combination, higher levels of this cytokine were observed. The highest TNF-alpha response was seen following stimulation with MFPML. MFP material not infected with ML did not elicit any response. The difference in TNF-alpha response shown by ADML and MFPML was postulated to be largely due to the presence of higher levels of PGL-I in MFPML. This increase in TNF-alpha production suggests that PGL-I may play a significant role in the induction of TNF-alpha during natural infection. | en_US |
| dc.description.affiliation | Leprosy Mission Nepal, Anandaban Hospital, Kathmandu, Nepal. | en_US |
| dc.identifier.citation | Dhungel S, Ranjit C, Sapkota BR, Macdonald M. Role of PGL-I of M. leprae in TNF-alpha production by in vitro whole blood assay. Nepal Medical College Journal. 2008 Mar; 10(1): 1-3 | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/46867 | |
| dc.language.iso | eng | en_US |
| dc.source.uri | https://www.nmcth.edu/list_journal_articles.php | en_US |
| dc.source.uri | https://www.nmcth.edu/nmcj_articles_pdf/volume_wise/nmcj_vol10_no1/sdhungel.pdf | en_US |
| dc.subject.mesh | Adolescent | en_US |
| dc.subject.mesh | Adult | en_US |
| dc.subject.mesh | Antigens, Bacterial --pharmacology | en_US |
| dc.subject.mesh | Blood Cells --metabolism | en_US |
| dc.subject.mesh | Glycolipids --pharmacology | en_US |
| dc.subject.mesh | Humans | en_US |
| dc.subject.mesh | Leprosy --metabolism | en_US |
| dc.subject.mesh | Male | en_US |
| dc.subject.mesh | Mycobacterium leprae | en_US |
| dc.subject.mesh | Tumor Necrosis Factor-alpha --biosynthesis | en_US |
| dc.title | Role of PGL-I of M. leprae in TNF-alpha production by in vitro whole blood assay. | en_US |
| dc.type | Journal Article | en_US |
| dc.type | Research Support, Non-U.S. Gov't | en_US |