Binding characteristics of sperm with recombinant human zona pellucida glycoprotein-3 coated beads.

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dc.contributor.authorBansal, Pankaj
dc.contributor.authorGupta, S K
dc.date.accessioned2011-12-14T05:38:52Z
dc.date.available2011-12-14T05:38:52Z
dc.date.issued2009-07
dc.description.abstractBackground & objectives: An inability or decreased ability of spermatozoa to bind to the zona pellucida (ZP), an extracellular glycoproteinaceous matrix surrounding egg, is one of the plausible causes of idiopathic infertility. It will be clinically useful to distinguish this condition from other causes of infertility. An assay system, investigating binding of human sperm with ZP glycoprotein may prove useful in this regard. We attempted to develop a simple assay system to analyse the binding of capacitated human spermatozoa to human zona pellucida glycoprotein-3 (ZP3) using baculovirus-expressed recombinant human ZP3 coated beads. Methods: Recombinant baculovirus-expressed ZP3 was purified, labelled with biotin and coated on streptavidin sepharose beads. An in vitro assay system was optimized to study binding of capacitated human sperm to ZP3 coated beads. Results: A higher percentage of baculovirus-expressed recombinant human ZP3 coated beads showed significant (P<0.05) binding of capacitated human sperm as compared to beads coated with fetuin. An inhibition in the binding of sperm to ZP3 coated beads was observed in presence of cold recombinant human ZP3. Further, prior incubation of ZP3 coated beads with monoclonal antibodies (MAbs) against ZP3 but not against ZP2 resulted in the decrease in number of sperm bound to bead. Interpretation & conclusion: An in vitro assay system to study the binding of human sperm to ZP3- primary sperm receptor was established, which may be useful to determine the functional competence of spermatozoa.en_US
dc.identifier.citationBansal Pankaj, Gupta S K. Binding characteristics of sperm with recombinant human zona pellucida glycoprotein-3 coated beads. Indian Journal of Medical Research. 2009 Jul; 130(1): 37-43.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135861
dc.language.isoenen_US
dc.source.urihttps://icmr.nic.in/ijmr/2009/july/0705.pdfen_US
dc.subjectBaculovirus-expression systemen_US
dc.subjecthuman zona pellucida glycoprotein-3en_US
dc.subjectmonoclonal antibodyen_US
dc.subjectspermatozoaen_US
dc.subjectstreptavidin sepharose beadsen_US
dc.subject.meshEgg Proteins --genetics
dc.subject.meshEgg Proteins --metabolism
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMembrane Glycoproteins --genetics
dc.subject.meshMembrane Glycoproteins --metabolism
dc.subject.meshProtein Binding
dc.subject.meshReceptors, Cell Surface --genetics
dc.subject.meshReceptors, Cell Surface --metabolism
dc.subject.meshRecombinant Fusion Proteins --genetics
dc.subject.meshRecombinant Fusion Proteins --metabolism
dc.subject.meshSperm Capacitation --physiology
dc.subject.meshSpermatozoa --cytology
dc.subject.meshSpermatozoa --metabolism
dc.subject.meshZona Pellucida --metabolism
dc.subject.meshalpha-Fetoproteins --metabolism
dc.titleBinding characteristics of sperm with recombinant human zona pellucida glycoprotein-3 coated beads.en_US
dc.typeArticleen_US
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