Reliable sex determination of mouse preimplantation embryos by PCR amplification of male-specific genes in single blastomeres.

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dc.contributor.authorTangchaisin, Pen_US
dc.contributor.authorPruksananonda, Ken_US
dc.contributor.authorTrirawatanapong, Ten_US
dc.contributor.authorVirutamasen, Pen_US
dc.date.accessioned2009-05-27T21:29:59Z
dc.date.available2009-05-27T21:29:59Z
dc.date.issued1996-12-01en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractOBJECTIVE: To assess the reliability of sex determination in mouse preimplantation embryos using the two-step polymerase chain reaction method. SETTING: Division of Immunology, Department of Microbiology and Division of Reproductive Medicine, Department of OB/GYN. METHODS: The Sry and Zfy genes, known to be present in the sex-determining region of mouse Y chromosome, were selected for Y-specific target sequences and DXNds 3 locus located on mouse X chromosome was served as the internal control sequence. DNAs extracted from heart blood of male and female mice were used to test the correctness and specificity of the selected primers using the two-step PCR method. The same experimental conditions were then used to amplify the single copy genes in single mouse blastomeres with two pairs of primers for each of the target sequences. The sex-determined embryos were transferred to the uteri of pseudopregnant recipients to test the consistency of the assay system. RESULTS: All male and female blood DNA sample results confirmed the correct sex identification of the origin (100%). Nineteen of 20 single blastomeres showed the accurate diagnosis when compared with theirs 7/8 embryos. The sex of 36 of 37 mouse pups born from biopsied male and female embryos agreed with the predicted sex. CONCLUSION: The reliable genetic analysis of sex chromosome- specific sequences in single cell is possible by the two-step PCR method and could be applied for diagnosis of defective genes of human preimplantation embryos derived from the in vitro fertilization program.en_US
dc.description.affiliationInterdepartment of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.en_US
dc.identifier.citationTangchaisin P, Pruksananonda K, Trirawatanapong T, Virutamasen P. Reliable sex determination of mouse preimplantation embryos by PCR amplification of male-specific genes in single blastomeres. Journal of the Medical Association of Thailand. 1996 Dec; 79 Suppl 1(): S78-87en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/44897
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlastocysten_US
dc.subject.meshBlastomeres --cytologyen_US
dc.subject.meshDNA Primersen_US
dc.subject.meshDNA-Binding Proteins --geneticsen_US
dc.subject.meshEmbryo Transferen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred ICRen_US
dc.subject.meshNuclear Proteinsen_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.subject.meshSex Determination (Analysis) --methodsen_US
dc.subject.meshSex-Determining Region Y Proteinen_US
dc.subject.meshTranscription Factorsen_US
dc.subject.meshY Chromosomeen_US
dc.titleReliable sex determination of mouse preimplantation embryos by PCR amplification of male-specific genes in single blastomeres.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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