Histological and radiographic evaluation of the muscle tissue of rats after implantation of bone morphogenic protein (rhBMP-2) in a scaffold of inorganic bone and after stimulation with low-power laser light.

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dc.contributor.authorBengtson, Nadya Galvão
dc.contributor.authorBengtson, Camilla Regina Galvão
dc.contributor.authorMendes, Fausto Medeiros
dc.contributor.authorPinheiro, Sérgio Luiz
dc.date.accessioned2012-07-27T03:21:17Z
dc.date.available2012-07-27T03:21:17Z
dc.date.issued2009-07
dc.description.abstractObjective: The present study histologically and radiologically evaluates the muscle tissue of rats after implantation of bone morphogenic protein (rhBMP-2) in a natural inorganic bone mineral scaffold from a bull calf femur and irradiation with low-power light laser. Materials and Methods: The right and left hind limbs of 16 rats were shaved and an incision was made in the muscle on the face corresponding to the median portion of the tibia, into which rhBMP-2 in a scaffold of inorganic bone was implanted. Two groups of limbs were formed: control (G1) and laser irradiation (G2). G2 received diode laser light applied in the direction of the implant, at a dose of 8 J/cm2 for three minutes. On the 7th, 21st, 40th and 112th days after implantation, hind limbs of 4 animals were radiographed and their implants removed together with the surrounding tissue for study under the microscope. The histological results were graded as 0=absence, 1=slight presence, 2=representative and 3=very representative, with regard to the following events: formation of osteoid structure, acute inflammation, chronic inflammation, fibrin deposition, neovascularization, foreign-body granuloma and fibrosis. Results: There were no statistically significant differences in these events at each evaluation times, between the two groups (P>0.05; Mann-Whitney test). Nevertheless, it could be concluded that the natural inorganic bone matrix with rhBMP-2, from the femur of a bull calf, is a biocompatible combination. Conclusions: Under these conditions, the inductive capacity of rhBMP-2 for cell differentiation was inhibited. There was a slight acceleration in tissue healing in the group that received irradiation with low-power laser light.en_US
dc.identifier.citationBengtson Antonio Lucindo, Bengtson Nadya Galvão, Bengtson Camilla Regina Galvão, Mendes Fausto Medeiros, Pinheiro Sérgio Luiz. Histological and radiographic evaluation of the muscle tissue of rats after implantation of bone morphogenic protein (rhBMP-2) in a scaffold of inorganic bone and after stimulation with low-power laser light. Indian Journal of Dental Research. 2010 Jul-Sept; 21(3): 420-424.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/139893
dc.language.isoenen_US
dc.source.urihttps://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=3;spage=420;epage=424;aulast=Bengtsonen_US
dc.subjectInorganic bone scaffolden_US
dc.subjectrhBMP-2en_US
dc.subjectlaser lighten_US
dc.subject.meshAbsorbable Implants
dc.subject.meshAnimals
dc.subject.meshBiocompatible Materials --therapeutic use
dc.subject.meshBone Matrix --drug effects
dc.subject.meshBone Matrix --radiation effects
dc.subject.meshBone Matrix --transplantation
dc.subject.meshBone Morphogenetic Protein 2
dc.subject.meshBone Morphogenetic Proteins --administration & dosage
dc.subject.meshBone Morphogenetic Proteins --radiation effects
dc.subject.meshBone Morphogenetic Proteins --therapeutic use
dc.subject.meshCattle
dc.subject.meshCell Differentiation --drug effects
dc.subject.meshCell Differentiation --radiation effects
dc.subject.meshFibrin --analysis
dc.subject.meshFibrosis
dc.subject.meshGranuloma, Foreign-Body --etiology
dc.subject.meshGranuloma, Foreign-Body --pathology
dc.subject.meshInflammation
dc.subject.meshLaser Therapy, Low-Level --methods
dc.subject.meshLasers, Semiconductor --therapeutic use
dc.subject.meshMale
dc.subject.meshMuscle, Skeletal --pathology
dc.subject.meshMuscle, Skeletal --radiography
dc.subject.meshMuscle, Skeletal --surgery
dc.subject.meshNeovascularization, Physiologic --drug effects
dc.subject.meshNeovascularization, Physiologic --radiation effects
dc.subject.meshOsteogenesis --drug effects
dc.subject.meshOsteogenesis --radiation effects
dc.subject.meshRadiation Dosage
dc.subject.meshRats
dc.subject.meshRats, Wistar
dc.subject.meshRecombinant Proteins --administration & dosage
dc.subject.meshRecombinant Proteins --radiation effects
dc.subject.meshRecombinant Proteins --therapeutic use
dc.subject.meshTime Factors
dc.subject.meshTissue Scaffolds
dc.subject.meshTransforming Growth Factor beta --administration & dosage
dc.subject.meshTransforming Growth Factor beta --radiation effects
dc.subject.meshTransforming Growth Factor beta --therapeutic use
dc.subject.meshWound Healing --drug effects
dc.subject.meshWound Healing --radiation effects
dc.titleHistological and radiographic evaluation of the muscle tissue of rats after implantation of bone morphogenic protein (rhBMP-2) in a scaffold of inorganic bone and after stimulation with low-power laser light.en_US
dc.typeArticleen_US
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