Flow cytometric analysis of Mixed phenotype acute leukemia: Experience from a tertiary oncology center.

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dc.contributor.authorSukumaran, Renu
dc.contributor.authorNair, Rekha A
dc.contributor.authorJacob, Priya Mary
dc.contributor.authorAnila, Kunjulekshmi Amma Raveendran Nair
dc.contributor.authorPrem, Shruthy
dc.contributor.authorBinitha, Rajeswary
dc.contributor.authorKusumakumary, Parukuttyamma
dc.date.accessioned2015-06-29T07:04:39Z
dc.date.available2015-06-29T07:04:39Z
dc.date.issued2015-04
dc.description.abstractIntroduction: Mixed phenotype acute leukemia (MPAL) is a rare subset of acute leukemia where the blasts exhibit lineage specifi c antigens of more than one lineage. Flow cytometric immunophenotyping is essential for the diagnosis of MPAL and the accurate diagnosis highly depends on the panel of markers used. The precise incidence of MPAL is uncertain as various institutions use different combinations of antibodies to assign the blasts to a particular lineage. Aim: The aim was to study the immunoprofi le of acute leukemia including aberrant antigen expressions and to study the incidence, clinical features, laboratory fi ndings, and immunophenotype of MPAL in our institution. Materials and Methods: All cases of acute leukemias in which fl ow cytometric analysis during 1-year period from July 2012 to July 2013 were included in this study. Results: During the study period, fl ow cytometric analysis of 506 cases was performed. B lymphoblastic leukemia was the most common subtype of acute leukemia. CD13 was the most common aberrant antigen expression in acute lymphoblastic leukemia and CD7 was the most common aberrant antigen expression in acute myeloid leukemia. A diagnosis of MPAL was made in 15 cases, which accounted for 2.96% of all leukemias. 9 cases were diagnosed as T/myeloid, 5 cases as B/myeloid and 1 case as B/T. Conclusion: Mixed phenotype acute leukemia is a rare subset of acute leukemia. Flow cytometry is critical in establishing a diagnosis of MPAL. The panel of antibodies used is important in the identifi cation of the “mixed” phenotype. Cytoplasmic markers (cytoplasmic MPO, cytoplasmic 79a, cytoplasmic 22 and cytoplasmic CD3) should be included in the primary flow cytometric panel.en_US
dc.identifier.citationSukumaran Renu, Nair Rekha A, Jacob Priya Mary, Anila Kunjulekshmi Amma Raveendran Nair, Prem Shruthy, Binitha Rajeswary, Kusumakumary Parukuttyamma. Flow cytometric analysis of Mixed phenotype acute leukemia: Experience from a tertiary oncology center. Indian Journal of Pathology & Microbiology. 2015 Apr-Jun 58(2): 181-186.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/158581
dc.language.isoenen_US
dc.source.urihttps://www.ijpmonline.org/article.asp?issn=0377-4929;year=2015;volume=58;issue=2;spage=181;epage=186;aulast=Sukumaranen_US
dc.subjectAberrant antigen expressionen_US
dc.subjectflow cytometryen_US
dc.subjectmixed phenotype acute leukemiaen_US
dc.titleFlow cytometric analysis of Mixed phenotype acute leukemia: Experience from a tertiary oncology center.en_US
dc.typeArticleen_US
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