Comparison of Cryptosporidium parvum development in various cell lines for screening in vitro drug testing.

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dc.contributor.authorSiripanth, Chutatipen_US
dc.contributor.authorPunpoowong, Benjaneeen_US
dc.contributor.authorAmarapal, Pornsawanen_US
dc.contributor.authorThima, Niramolen_US
dc.contributor.authorEampokalap, Boonchuayen_US
dc.contributor.authorKaewkungwal, Joraniten_US
dc.date.accessioned2009-05-27T15:58:32Z
dc.date.available2009-05-27T15:58:32Z
dc.date.issued2004-09-04en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThis study describes the development of Cryptosporidium parvum in MDCK, MA-104, Hep-2 and Vero cell lines. Differences in susceptibility, infectivity, and the methodology of excystation were determined. Various solutions were considered to determine the factors which enhanced the excystation (eg with and without sodium hypochlorite, trypsin or sodium taurocholate). It was shown that the sporozoites could be excysted in media either with or without trypsin and sodium taurocholate, but the number of sporozoites in the latter solution was less than the former one. Only oocysts digested by sodium hypochlorite and trypsin can enter the culture cells. Numerous meronts and oocysts were demonstrated and persisted for 9 days. Asexual stages were not observed in MA-104. Only few oocysts could be detected 1-3 days post-inoculation. There was a significant difference between the number of oocysts, which invaded MDCK, MA-104, and Hep-2 cells. MDCK gave the highest susceptibility to oocyst invasion among the three cell lines and asexual stages were also found. Among the 25 isolates, which had been cultivated, 23 isolates could infect MDCK and Hep-2. Only 2 isolates could not infect the MDCK cell. These 2 isolates could infect the Vero cell and yielded high numbers of trophozoites. Praziquantel (PZQ), doxycycline, and paromomycin (PRM) were tested on the infecting parasites. The drugs were added either with the inoculum or 24 hours after inoculation. None of them was effective, including PRM, which had been previously reported as effective.en_US
dc.description.affiliationDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. tmcsr@mahidol.ac.then_US
dc.identifier.citationSiripanth C, Punpoowong B, Amarapal P, Thima N, Eampokalap B, Kaewkungwal J. Comparison of Cryptosporidium parvum development in various cell lines for screening in vitro drug testing. The Southeast Asian Journal of Tropical Medicine and Public Health. 2004 Sep; 35(3): 540-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34115
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2004_35_3/08-3284.pdfen_US
dc.subject.meshAcquired Immunodeficiency Syndrome --complicationsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnthelmintics --administration & dosageen_US
dc.subject.meshAnti-Bacterial Agents --administration & dosageen_US
dc.subject.meshCell Culture Techniquesen_US
dc.subject.meshCell Line --drug effectsen_US
dc.subject.meshCryptosporidiosis --complicationsen_US
dc.subject.meshCryptosporidium parvum --drug effectsen_US
dc.subject.meshFeces --parasitologyen_US
dc.subject.meshHumansen_US
dc.subject.meshOocysts --drug effectsen_US
dc.subject.meshSodium Hypochlorite --pharmacologyen_US
dc.subject.meshSporozoites --drug effectsen_US
dc.subject.meshTaurocholic Acid --pharmacologyen_US
dc.subject.meshTrypsin --pharmacologyen_US
dc.titleComparison of Cryptosporidium parvum development in various cell lines for screening in vitro drug testing.en_US
dc.typeComparative Studyen_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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