Active Case Detection and Prevalence of Urinary Schistosomiasis and Malaria in Pupils of Kotto Barombi, Southwest Cameroon Using the CyScope® Fluorescence Microscope.
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Date
2015
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Abstract
Aim: This study was aimed at assessing the use of the CyScope® fluorescence microscope to
determine the prevalence of urinary schistosomiasis (US) and malaria in Kotto Barombi.
Experimental Design: The study was a cross-sectional survey.
Place and Duration of Study: The study was carried out in Kotto Barombi, Cameroon from April to
May, 2013.
Methodology: Urine and blood samples were collected from 216 pupils. US eggs were detected in
urine by centrifugation and CyScope® methods for schistosome eggs. Malaria parasites were
detected using Giemsa-stained blood films and CyScope® methods. The performance
characteristics of the CyScope® for both infections were determined using light microscopy as gold
standard.
Results: Overall prevalence of US was 43.4% and 48.5% by light microscopy and CyScope®
respectively. Prevalence of US was significantly higher (P<0.01) in the Kotto Barombi Island
(78.3%) than Mainland (33.8%). US prevalence was not affected by age, sex and socio-economic
class (SEC). Mean intensity of US was 8.1 eggs/10 ml urine (Confidence interval, CI = 4.3–11.9). It
was significantly higher (P =.01) in pupils from Island (36.5 eggs/10 ml, CI: 17.7–55.3) than
Mainland (8.8 eggs/10 ml; 7.1–10.5), males (19.2 eggs/10 ml urine; CI: 9.2–29.2) than females
(17.8 eggs/10 ml urine; CI: 13.1–22.5) and highest (P = .046) in the ≤6 years age group (36.9
eggs/10 ml; CI: 20.4–53.4) when compared with pupils in other age groups. Sensitivity and
specificity of CyScope® for US were 90.6% and 83.8% respectively. Overall prevalence of malaria
was 19.0% and 41.2% by light microscopy and CyScope® respectively and the difference was
significant (P = .01). Malaria prevalence and density were not influenced by age; sex and SEC.
Sensitivity and specificity of CyScope® for malaria were 68.3% and 64.9% respectively.
Conclusion: The CyScope® could be a useful tool for active case detection of both diseases
especially in areas that lack electricity.
Description
Keywords
Malaria;, Urinary schistosomiasis, diagnosis, pupils, Partec CyScope, Kotto Barombi
Citation
Kimbi Helen Kuokuo, Wepnje Godlove B, Anchang-Kimbi Judith, Tonga Calvin, Ayukenchengamba Bate, Njabi Conica, Nono Larissa Kouodjip, Nyabeyeu Hervé Nyabeyeu, Lehman Leopold Gustave. Active Case Detection and Prevalence of Urinary Schistosomiasis and Malaria in Pupils of Kotto Barombi, Southwest Cameroon Using the CyScope® Fluorescence Microscope. International Journal of TROPICAL DISEASE & Health. 2015; 8(1): 1-12.