Assessment of DNA nucleo base oxidation and antioxidant defense in postmenopausal women under hormone replacement therapy.

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dc.contributor.authorAkcay, Tülay
dc.contributor.authorDincer, Yildiz
dc.contributor.authorSaygili, E Ilker
dc.contributor.authorSeyisoglu, Hakan
dc.contributor.authorErtungalp, Erdogan
dc.date.accessioned2013-03-06T10:04:13Z
dc.date.available2013-03-06T10:04:13Z
dc.date.issued2010-01
dc.description.abstractBackground and Objective: The aim of the present study was to evaluate oxidative stress byinvesting oxidatively damaged DNA AS Formamidopyrimidine DNA glycosylase (Fpg) -sensitive sites, glutathione peroxidase (GPx), superoxide dismutase (SOD) activities reduced glutathione (GSH) level and nitrite level as satble end product of in women receiving hormone replacement therapy (HRT). Materials and Methods: 127 healthy postmenopausal women receiving HRT and 25 healthy control postmenopausal women were included in this study. Women receiving HRT, comprised surgical menopausal women who underwent surgery for benign conditionsand received conjugated equine estrogen, 0.625 mg/day for 1year (group 1), 5 years (group 2) and more than 10 years (group 3), spontaneous postmenopausal women received conjugated equine estrogen, 0.625 (Premarin) mg/day and medroxyprogesterone acetate, 2.5 mg/day (Premelle) for 1 year (group 4), 5 years (group 5) and more than 5 years (group 6).We investigated in the present study the effects of HRT on nitrite level and GSH level, activities of SOD and GPx and oxidative damage to DNA by comet assays by measuring levels of Fpg-sensitive sites. Results: Although no significant differences were found in the SOD activities, in total group receiving HRT, increased DNA oxidation (P<0.001) together with an increased GPx activity (P<0.001) and nitrite level (P<0.001) as well as a decreased GSH level (P < 0.05) as compared with controls were observed. Conclusion: Estrogen alone or oestrogen in combination with progesterone and duration of use did not significantly alter the results. We evaluated that caused oxidative stress by investigating oxidative DNA damage as Fp-sensitive sites and GSH.NO levels in women receiving HRT.en_US
dc.identifier.citationAkcay Tülay, Dincer Yildiz, Saygili E Ilker, Seyisoglu Hakan, Ertungalp Erdogan. Assessment of DNA nucleo base oxidation and antioxidant defense in postmenopausal women under hormone replacement therapy. Indian Journal of Medical Sciences. 2010 Jan; 64(1) 17-25.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/145477
dc.language.isoenen_US
dc.source.urihttps://www.indianjmedsci.org/article.asp?issn=0019-5359;year=2010;volume=64;issue=1;spage=17;epage=25;aulast=Akcayen_US
dc.subject8-Hydroxy -2′-deoxyguanosineen_US
dc.subjectestrogenen_US
dc.subjectglutathioneen_US
dc.subjectglutathioneperoxidaseen_US
dc.subjectnitrite superoxide dismutaseen_US
dc.subject.lcshFemale
dc.subject.meshAnalysis of Variance
dc.subject.meshAntioxidants --metabolism
dc.subject.meshCase-Control Studies
dc.subject.meshDNA Damage --drug effects
dc.subject.meshDNA-Formamidopyrimidine Glycosylase --metabolism
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshDrug Administration Schedule
dc.subject.meshEstrogen Replacement Therapy --adverse effects
dc.subject.meshEstrogen Replacement Therapy --methods
dc.subject.meshEstrogens, Conjugated (USP) --administration & dosage
dc.subject.meshEstrogens, Conjugated (USP) --adverse effects
dc.subject.meshFollow-Up Studies
dc.subject.meshHormone Replacement Therapy --adverse effects
dc.subject.meshHormone Replacement Therapy --methods
dc.subject.meshHumans
dc.subject.meshMedroxyprogesterone Acetate --administration & dosage
dc.subject.meshMedroxyprogesterone Acetate --adverse effects
dc.subject.meshMiddle Aged
dc.subject.meshOxidation-Reduction --drug effects
dc.subject.meshOxidative Stress --drug effects
dc.subject.meshPostmenopause --blood
dc.subject.meshPostmenopause --drug effects
dc.subject.meshReference Values
dc.subject.meshTreatment Outcome
dc.titleAssessment of DNA nucleo base oxidation and antioxidant defense in postmenopausal women under hormone replacement therapy.en_US
dc.typeArticleen_US
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