Studies on the development of DNA vaccine against Cysticercus cellulosae infection and its efficacy.

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dc.contributor.authorCai, Xen_US
dc.contributor.authorChai, Zen_US
dc.contributor.authorJing, Zen_US
dc.contributor.authorWang, Pen_US
dc.contributor.authorLuo, Xen_US
dc.contributor.authorChen, Jen_US
dc.contributor.authorDou, Yen_US
dc.contributor.authorFeng, Sen_US
dc.contributor.authorSu, Cen_US
dc.contributor.authorJin, Jen_US
dc.date.accessioned2009-05-27T14:38:22Z
dc.date.available2009-05-27T14:38:22Z
dc.date.issued2001-03-04en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractDNA vaccine against Cysticercus cellulosae infection was developed and its efficacy was tested. A pair of primers specific to antigen B gene of C. cellulosae was designed which amplified the gene successfully with RT-PCR. The gene was ligated to PV93 vector, and the recombinant of antigen B gene and PV93 was transformed to JM83 cells. The transformed JM83 cells were cultured in a large scale and the plasmid purified. Based on the recombinant plasmid. a DNA vaccine was developed and used to vaccinate two groups of experimental pigs. In each group, there was a routine vaccine, an enhanced vaccine and a control group. Groups 1 and 2 were challenged at 4 months and at 14 days post vaccination respectively with eggs of Taenia solium. The antibody response was also tested with ELISA. The results suggested that all animals vaccinated AgB gene DNA vaccine, no matter by routine or enhanced vaccine, their antibodies reached maximum peak 23 days post vaccination and decreased gradually. When the animals were challenged 4 months after vaccination, they had strong immunity and the parasites decrease rates were 91.2% and 93.1% respectively. When pigs vaccinated with AgB gene DNA vaccine were challenged 14 days post vaccination with 18,000 eggs/pig. The animals showed strong immunity and the parasite decrease rates were 99.5% and 84.9% respectively. However at that time, the antibodies did not reach the peak. While in the control group, the number of C. cellulosae was as many as 2,500. It was concluded that the pigs vaccinated with DNA vaccine had strong immunity against infection of eggs of T. solium.en_US
dc.description.affiliationLanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Gansu, People's Republic of China. caixp@public.lz.gs.cnen_US
dc.identifier.citationCai X, Chai Z, Jing Z, Wang P, Luo X, Chen J, Dou Y, Feng S, Su C, Jin J. Studies on the development of DNA vaccine against Cysticercus cellulosae infection and its efficacy. The Southeast Asian Journal of Tropical Medicine and Public Health. 2001 ; 32 Suppl 2(): 105-10en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/30675
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Helminth --biosynthesisen_US
dc.subject.meshAntigens, Helminth --geneticsen_US
dc.subject.meshCysticercosis --prevention & controlen_US
dc.subject.meshCysticercus --geneticsen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --veterinaryen_US
dc.subject.meshMeat --parasitologyen_US
dc.subject.meshRNA, Helminth --chemistryen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSwineen_US
dc.subject.meshSwine Diseases --parasitologyen_US
dc.subject.meshTreatment Outcomeen_US
dc.subject.meshVaccination --standardsen_US
dc.subject.meshVaccines, DNA --administration & dosageen_US
dc.subject.meshZoonosesen_US
dc.titleStudies on the development of DNA vaccine against Cysticercus cellulosae infection and its efficacy.en_US
dc.typeJournal Articleen_US
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