Effect of dilution of 5% vinegar on Candida albicans
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2010-11-03
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Khon Kaen University Dental Journal
Abstract
The study of effect of dilution of 5% vinegar and soaking time to reduction of the colony-formings of Candidia albicans was done. One hundred and eighty heat cured acrylic plates size of 2 mm x 7 mm x 2 mm (N=180) were contaminated with Candidia albicans. The samples were separated into 10 set of 18. Nine plates of each set were soaked separately in 9 test groups, distilled water, 5.25% sodium hypochlorite, 5% vinegar, 5% vinegar diluted with distilled water 1:1, 1:2, 1:4, 1:6, 1:8 and 1:10 for 1 minute and the other nine for 3 minutes. The grossly adherent cells of Candidia albicans on the samples were removed by phosphate buffer saline solution. Then the samples were vibrated in phosphate buffer saline solution for 1 minute. These phosphate buffer saline solutions were diluted and then incubated on Sabouraud agar at 37° C for 48 hours. The colonies were counted and statistic analysis was performed by two-way ANOVA. The result showed that 5% vinegar and 1:1-1:6 dilutions of vinegar reduced colony-forming units of Candidia albicans more than distilled water significantly (P-value
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Khon Kaen University Dental Journal; Vo.12 No. 1 Jan - June 2009