Identification of natural compounds which inhibit biofilm formation in clinical isolates of Klebsiella pneumoniae.

Simple item page
dc.contributor.authorMagesh, H
dc.contributor.authorKumar, Arun
dc.contributor.authorAlam, Ayesha
dc.contributor.authorPriyam, Priyam
dc.contributor.authorSekar, Uma
dc.contributor.authorSumantran, Venil N
dc.contributor.authorVaidyanathan, Rama
dc.date.accessioned2013-11-19T04:52:20Z
dc.date.available2013-11-19T04:52:20Z
dc.date.issued2013-09
dc.description.abstractKlebsiella pneumoniae, an important opportunistic pathogen, exists as a biofilm in persistent infections and in-dwelling medical devices. With the objective of identifying natural compounds inhibiting biofilm formation in K. pneumoniae, 35clinical isolates were screened,out of which 7 strong biofilm producers were identified. Six natural compounds were tested for their inhibitory effects on bacterial growth and biofilm formation by determining the minimum inhibitory concentration and minimum concentration for biofilm inhibition (MBIC) for each compound. The results show that reserpine followed by linoleic acid, were the most potent biofilm inhibitors. Reserpine, an efflux pump inhibitor was effective at biofilm inhibition at a concentration of 0.0156 mg/mL, 64-fold lower concentration than its MIC. Linoleic acid, an essential fatty acid was effective as a biofilm inhibitor at 0.0312 mg/mL, which is 32-fold lower than its MIC. Berberine, another plant derived antimicrobial, chitosan and eugenol had an MBIC value of 0.0635 mg/mL. Curcumin, a natural phenolic compound was effective at biofilm inhibition at a concentration of 0.25 mg/mL, which is 50 fold less than its MIC. Notably, the MIC and MBIC data on these 6 natural compounds was reproducible in all seven high biofilm forming isolates of K. pneumoniae. The present report is a comprehensive comparative analysis of the dose dependent inhibition of various natural compounds on biofilm formation in K. pneumoniae.en_US
dc.identifier.citationMagesh H, Kumar Arun, Alam Ayesha, Priyam, Sekar Uma, Sumantran Venil N, Vaidyanathan Rama. Identification of natural compounds which inhibit biofilm formation in clinical isolates of Klebsiella pneumoniae. Indian Journal of Experimental Biology. 2013 Sept; 51(9): 764-772.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/149381
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/21073en_US
dc.subjectBiofilm inhibitionen_US
dc.subjectEfflux pumps inhibitorsen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectMulti-drug resistanceen_US
dc.subjectMinimum inhibitory concentrationen_US
dc.subject.meshBiofilms --drug effects
dc.subject.meshBiological Products --pharmacology
dc.subject.meshKlebsiella pneumoniae --drug effects
dc.subject.meshKlebsiella pneumoniae --growth & development
dc.subject.meshKlebsiella pneumoniae --physiology
dc.subject.meshMicrobial Sensitivity Tests
dc.titleIdentification of natural compounds which inhibit biofilm formation in clinical isolates of Klebsiella pneumoniae.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijeb2013v51n9p764.pdf
Size:
622.07 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Experimental Biology