Differential diagnosis of non-small cell lung carcinoma by circulating microRNA

dc.contributor.authorSingh, Anjanaen_US
dc.contributor.authorKant, Ravien_US
dc.contributor.authorSaluja, Tajindra Singhen_US
dc.contributor.authorTripathi, Tanyaen_US
dc.contributor.authorSrivastava, Kaminien_US
dc.contributor.authorNaithani, Manishaen_US
dc.contributor.authorGupta, Anuragen_US
dc.contributor.authorMirza, Anissa Atifen_US
dc.contributor.authorPrakash, Veden_US
dc.contributor.authorSingh, Satyendra Kumaren_US
dc.date.accessioned2020-11-18T10:08:16Z
dc.date.available2020-11-18T10:08:16Z
dc.date.issued2020-04
dc.description.abstractIntroduction: More than 70% of lung cancer comprises nonsmall-cell lung carcinoma and is associated with poor survival outcome owing to late diagnosis. Identification of lung cancer in early stages when no clinical signs or symptoms are evident, can drastically improve the prognosis. To this end, we aimed to evaluate the changes occurring at tissue level by assessing the expression of six microRNAs (miRNAs) in lung adenocarcinoma (AC) and squamous cell carcinoma (SCC). Materials and Methods: Peripheral blood of histopathologically proven cases of lung AC and SCC was collected and processed for the isolation of miRNAs using commercially available kit. Primers against mir-2114, mir-2115, mir-2116, mir-2117, mir-449c, and mir-548q with loading control Caenorhabditis elegans were used. Screening was carried out in thirty cases of both AC and SCC, whereas twenty healthy controls were included. Results:Real-time polymerase chain reaction data revealed that the expression of mir-2114 and mir-449c in AC and mir-2115 in SCC was significantly upregulated. The expression of these miRNAs was also confirmed in lung AC cell line. The differential pattern of expression of these miRNAs can be used for precise diagnosis of lung carcinoma Conclusions: We have used a noninvasive technique to identify the subtype of lung cancer based on molecular genetic signatures. The results suggest that through molecular profiling of miRNA, we can screen high-risk cases for cancer interceptionen_US
dc.identifier.affiliationsDepartment of Biochemistry, AIIMS, Rishikesh, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, AIIMS, Rishikesh, Indiaen_US
dc.identifier.affiliationsCenter For Advance Research, Stem Cell/Cell Culture Lab, King George's Medical University, Lucknow, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsCenter For Advance Research, Stem Cell/Cell Culture Lab, King George's Medical University, Lucknow, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsCenter For Advance Research, Stem Cell/Cell Culture Lab, King George's Medical University, Lucknow, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, AIIMS, Rishikesh, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, AIIMS, Rishikesh, Indiaen_US
dc.identifier.affiliationsCenter For Advance Research, Stem Cell/Cell Culture Lab, King George's Medical University, Lucknow, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsDepartment of Pulmonary Critical Care, King George's Medical University, Lucknow, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsCenter For Advance Research, Stem Cell/Cell Culture Lab, King George's Medical University, Lucknow, Uttar Pradesh, Indiaen_US
dc.identifier.citationSingh Anjana, Kant Ravi, Saluja Tajindra Singh, Tripathi Tanya, Srivastava Kamini, Naithani Manisha, Gupta Anurag, Mirza Anissa Atif, Prakash Ved, Singh Satyendra Kumar. Differential diagnosis of non-small cell lung carcinoma by circulating microRNA. Journal of Cancer Research and Therapeutics. 2020 Apr; 16(1): 127-131en_US
dc.identifier.issn0973-1482
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/213782
dc.languageenen_US
dc.publisherWolters Kluwer India Pvt. Ltd.en_US
dc.relation.issuenumber1en_US
dc.relation.volume16en_US
dc.source.urihttps://dx.doi.org//10.4103/jcrt.JCRT_872_19en_US
dc.subjectMicroRNAen_US
dc.subjectmolecular diagnosisen_US
dc.subjectnonsmall‑cell lung canceren_US
dc.subjectreal‑time polymerase chain reactionen_US
dc.titleDifferential diagnosis of non-small cell lung carcinoma by circulating microRNAen_US
dc.typeJournal Articleen_US
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