Vitamin A deficiency increases the oleic acid (C18:1) levels in the kidney of high fructose diet-fed rats

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dc.contributor.authorGopal, Reddy, MRen_US
dc.contributor.authorKumar, MSen_US
dc.contributor.authorAcharya, Ven_US
dc.contributor.authorVenkata, SMen_US
dc.contributor.authorPutcha, UKen_US
dc.contributor.authorJeyakumar, SMen_US
dc.date.accessioned2020-04-10T01:44:55Z
dc.date.available2020-04-10T01:44:55Z
dc.date.issued2019-12
dc.description.abstractBackground & objectives: Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme responsible for endogenous synthesis of monounsaturated fatty acids (MUFA) and plays a key role in various pathophysiology, including fatty liver diseases. In this experimental study the impact of vitamin A deficiency was assessed on SCD1 regulation in relation to kidney biology, under high fructose (HFr) diet-fed condition in rats. Methods: Forty male weanling (21 day old) Wistar rats were divided into four groups control, vitamin A-deficient (VAD), HFr, VAD with HFr consisting of eight rats each, except 16 for the VAD group. The groups received one of the following diets: control, VAD, HFr and VAD with HFr for 16 wk, except half of the VAD diet-fed rats were shifted to HFr diet, after eight week period. Results: Feeding of VAD diet (alone or with HFr) significantly reduced the kidney retinol (0.51, 0.44 ?g/g vs. 2.1 ?g/g; P < 0.05), while increased oleic (C18:1) and total MUFA levels (23.3, 22.2% and 27.3, 25.4% respectively vs. 14.7 and 16.6%; P < 0.05) without affecting the SCD1, both at protein and mRNA levels, when compared with HFr. Comparable, immunohistological staining for SCD1 was observed in the distal convoluted tubules. Despite an increase in MUFA, morphology, triglyceride content and markers of kidney function were not affected by VAD diet feeding. Interpretation & conclusions: Feeding of VAD diet either alone or under HFr condition increased the kidney oleic acid (C18:1) levels and thus total MUFA, which corroborated with elevated SCD1 activity index, without affecting its expression status. However, these changes did not alter the kidney morphology and function. Thus, nutrient-gene regulation in kidney biology seems to be divergent.en_US
dc.identifier.affiliationsDivisions of Lipid Biochemistry, ICMR-National Institute of Nutrition, Hyderabad, Telangana, Indiaen_US
dc.identifier.affiliationsDivisions of Pathology, ICMR-National Institute of Nutrition, Hyderabad, Telangana, Indiaen_US
dc.identifier.citationGopal Reddy MR, Kumar MS, Acharya V, Venkata SM, Putcha UK, Jeyakumar SM. Vitamin A deficiency increases the oleic acid (C18:1) levels in the kidney of high fructose diet-fed rats. Indian Journal of Medical Research. 2019 Dec; 150(6): 620-629en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/196084
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber6en_US
dc.relation.volume150en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_1574_17en_US
dc.subjectDesaturaseen_US
dc.subjectinflammationen_US
dc.subjectinjuryen_US
dc.subjectlipidsen_US
dc.subjectoleic aciden_US
dc.subjectoxidative stressen_US
dc.subjectsteatosisen_US
dc.subjectVADen_US
dc.titleVitamin A deficiency increases the oleic acid (C18:1) levels in the kidney of high fructose diet-fed ratsen_US
dc.typeJournal Articleen_US
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