Use of GenoType® MTBDRplus assay to assess drug resistance and mutation patterns of multidrug-resistant tuberculosis isolates in northern India.

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dc.contributor.authorMaurya, A K
dc.contributor.authorSingh, A K
dc.contributor.authorKant, S
dc.contributor.authorUmrao, J
dc.contributor.authorKumar, M
dc.contributor.authorKushwaha, R A S
dc.contributor.authorNag, V L
dc.contributor.authorDhole, T N
dc.date.accessioned2013-09-04T04:22:19Z
dc.date.available2013-09-04T04:22:19Z
dc.date.issued2013-07
dc.description.abstractPurpose: The emergence and spread of multidrug-resistant tuberculosis (MDR-TB) is a major public health problem. The diagnosis of MDR-TB is of paramount importance in establishing appropriate clinical management and infection control measures. The aim of this study was to evaluate drug resistance and mutational patterns in clinical isolates MDR-TB by GenoType® MTBDRplus assay. Material and Methods: A total of 350 non-repeated sputum specimens were collected from highly suspected drug-resistant pulmonary tuberculosis (PTB) cases; which were processed by microscopy, culture, differentiation and first line drug susceptibility testing (DST) using BacT/ALERT 3D system. Results: Among a total of 125 mycobacterium tuberculosis complex (MTBC) strains, readable results were obtained from 120 (96%) strains by GenoType® MTBDRplus assay. Only 45 MDR-TB isolates were analysed for the performance, frequency and mutational patterns by GenoType® MTBDRplus assay. The sensitivity of the GenoType® MDRTBplus assay for detecting individual resistance to rifampicin (RIF), isoniazid (INH) and multidrug resistance was found to be 95.8%, 96.3% and 97.7%, respectively. Mutation in codon S531L of the rpoB gene and codon S315T1 of katG genes were dominated in MDR-TB strains, respectively (P < 0.05). Conclusions: The GenoType® MTBDRplus assay is highly sensitive with short turnaround times and a rapid test for the detection of the most common mutations conferring resistance in MDR-TB strains that can readily be included in a routine laboratory workflow.en_US
dc.identifier.citationMaurya A K, Singh A K, Kant S, Umrao J, Kumar M, Kushwaha R A S, Nag V L, Dhole T N. Use of GenoType® MTBDRplus assay to assess drug resistance and mutation patterns of multidrug-resistant tuberculosis isolates in northern India. Indian Journal of Medical Microbiology. 2013 Jul-Sept; 31(3): 230-236.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/148089
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2013;volume=31;issue=3;spage=230;epage=236;aulast=Mauryaen_US
dc.subjectGenoType® MTBDRplus assayen_US
dc.subjectMDR-TBen_US
dc.subjectM. tuberculosis complexen_US
dc.subjecttuberculosisen_US
dc.titleUse of GenoType® MTBDRplus assay to assess drug resistance and mutation patterns of multidrug-resistant tuberculosis isolates in northern India.en_US
dc.typeArticleen_US
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