Aryl beta-galactosidase from Sclerotium rolfsii: physiological and biochemical studies.

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dc.contributor.authorBhosale, J Hen_US
dc.contributor.authorShewale, J Gen_US
dc.date.accessioned1995-02-01en_US
dc.date.accessioned2009-05-27T04:14:10Z
dc.date.available1995-02-01en_US
dc.date.available2009-05-27T04:14:10Z
dc.date.issued1995-02-01en_US
dc.description.abstractProduction of beta-galactosidase by Sclerotium rolfsii NCIM 1084 was studied under submerged fermentation conditions. The enzyme was produced extracellularly and constitutively on glucose. The enzyme production was enhanced when galactose, raffinose, cellobiose, sucrose, xylose, maltose, cellulose and pectin were used as carbon sources. Cellulose and diammonium hydrogen phosphate were best carbon and nitrogen sources, respectively. Surfactants such as Sag, Paraffin oil, Tween 20 and Tween 80 increased the enzyme production. Maximum yield of beta-galactosidase obtained was 3.8-4.2 nkat/ml. The optimum pH, optimum temperature and molecular weight of the beta-glactosidase were 2.7, 60 degrees C and 2,21,000 daltons, respectively. The enzyme is an aryl beta-glactosidase and did not hydrolyse lactose. The Km value for o-nitrophenyl beta-D-galactoside was 3.7 mM. Galactose and 2-mercaptoethanol inhibited the enzyme.en_US
dc.description.affiliationResearch and Development, Hindustan Antibiotics Ltd., Pimpri, Pune, India.en_US
dc.identifier.citationBhosale JH, Shewale JG. Aryl beta-galactosidase from Sclerotium rolfsii: physiological and biochemical studies. Hindustan Antibiotics Bulletin. 1995 Feb-Nov; 37(1-4): 25-36en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/2138
dc.language.isoengen_US
dc.subject.meshAscomycota --enzymologyen_US
dc.subject.meshCarbon --metabolismen_US
dc.subject.meshCellobiose --chemistryen_US
dc.subject.meshCellulose --chemistryen_US
dc.subject.meshFermentationen_US
dc.subject.meshGalactose --chemistryen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshHydrolysisen_US
dc.subject.meshLactose --metabolismen_US
dc.subject.meshMaltose --chemistryen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshPectins --chemistryen_US
dc.subject.meshPolysaccharides --chemistryen_US
dc.subject.meshQuaternary Ammonium Compounds --chemistryen_US
dc.subject.meshRaffinose --chemistryen_US
dc.subject.meshSucrose --chemistryen_US
dc.subject.meshSurface-Active Agents --chemistryen_US
dc.subject.meshTemperatureen_US
dc.subject.meshXylose --chemistryen_US
dc.subject.meshbeta-Galactosidase --analysisen_US
dc.titleAryl beta-galactosidase from Sclerotium rolfsii: physiological and biochemical studies.en_US
dc.typeJournal Articleen_US
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