A Preliminary study of the invertase activity in coconut

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dc.contributor.authorALLES, NHen_US
dc.date.accessioned2011-02-14T16:27:58Z
dc.date.available2011-02-14T16:27:58Z
dc.date.created1977en_US
dc.date.issued1977en_US
dc.descriptionDissertation: M.Sc., University of Sri Lanka (Colombo Campus): UC(MED), 1977.en_US
dc.description.abstractAB : A ` particulate ` invertase preparation of low activity (sedimenting in the 900 g fraction) was extracted from the stalk and mesocarp tissues of coconut (Cocos nucifera L.). The activity o invertase was found to increase linearly with the time of incubation (0 - 3h). Initial velocity was directly proportional to enzyme concentration, only in the low concentration ranges. The initial velocity decreased at enzyme concentrations higher than 0.13 mg protein per 2.0 ml of incubation mixture. Treatment of the particulate enzyme with 0.1 present and 0.5 present (v/v) of the nonionic surface active agent, Triton X - 100, solubilized 71 percent and 76 percent of the invertase. Incorporation of the non-acidic thiol, mercaptoethanol into the reaction mixture, caused significant activation of the invertase. This suggested the possibility of an SH group participating in the catalytic activity of the enzyme. In addition, mercaptoethanol may cause enzyme reactivation by reducing the quinones formed by the oxidation of polyphenols. Consequently, the low activity observed in the absence of mercaptoethanol was probally due in part, to an inactivation of the enzyme by quinones. The enzyme was found to be inactivated by the metal chelating agent, ethylene diamine tetracetate (EDTA) at 5 X 10-3 and 5 X 10-4 M concentrations. This finding suggested that invertase is probably a metalloenzyme. It was also found that the inactivation of the enzyme by EDTA (10-2 M) was not reversed by mercaptoethanol. This may indicate that the removal of the metal in question by EDTA, causes an irreversible denaturation of the enzyme. Polyphenols found in the enzyme preparation were found to interfere with the reduction of the dinitrosalicylic acid reagent. The use of insoluble polyclar in the extracting medium to remove these interfering substances, is suggested.en_US
dc.identifier.citationALLES, NH, A Preliminary study of the invertase activity in coconut, University of Sri Lanka (Colombo Campus) UC(MED), 1977: 47p.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/129785
dc.language.isoen_USen_US
dc.publisherUniversity of Sri Lanka (Colombo Campus): UC(MED).en_US
dc.rightsUniversity of Sri Lanka (Colombo Campus), UC(MED): Sri Lanka HELLIS Networken_US
dc.source.urihttps://hellis.srilanka.healthrepository.orgen_US
dc.subjectBeta-fructofuranosidaseen_US
dc.subject.meshbeta-Fructofuranosidaseen_US
dc.subject.meshbeta-Fructofuranosidase-isolation \& purificationen_US
dc.subject.meshbeta-Fructofuranosidase-chemistryen_US
dc.titleA Preliminary study of the invertase activity in coconuten_US
dc.typeThesisen_US
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