Utility of Detecting sof Gene as Evidence of Streptococcus pyogenes Infection in Acute Rheumatic Fever

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dc.contributor.authorAgarwal, Nikkien_US
dc.contributor.authorKapoor, Seemaen_US
dc.contributor.authorMangla, Ankiten_US
dc.contributor.authorKumar, Ashoken_US
dc.contributor.authorMandal, Ravi Nen_US
dc.contributor.authorKumar, Namita Ravien_US
dc.contributor.authorRamachandran, Mohanarajen_US
dc.date.accessioned2020-04-23T07:43:56Z
dc.date.available2020-04-23T07:43:56Z
dc.date.issued2019-04
dc.description.abstractObjectives: To determine the diagnostic accuracy of polymerase chain reaction-baseddetection of sof gene compared to throat swab culture for S. pyogenes infection in patientswith acute rheumatic fever and those with recurrence of rheumatic activity. Methods: 40patients between 3 to 18 years of age, with clinical diagnosis of acute rheumatic fever or newactivity in established rheumatic heart disease were included. The amplicon of 228bp of sofgene was detected using a polymerase chain reaction-based technique and the results werecompared with throat swab culture for Streptococcus pyogenes. Results: 10 patients hada positive throat swab culture and 11 had sof gene detected. The sensitivity and specificityof the test was 100% and 96.7%, respectively compared to throat swab culture (P=0.001).The positive predictive value and the negative predictive value was 90.9% and 100%respectively. Conclusions: Polymerase chain reaction-based detection of sof geneprovides an alternative to throat swab culture in diagnosing activity in Acute RheumaticFever or established Rheumatic heart disease.en_US
dc.identifier.affiliationsDepartment of Pediatrics, Maulana Azad Medical College, New Delhien_US
dc.identifier.affiliationsCase Western Reserve University School ofMedicine, Cleveland, Ohio, United Statesen_US
dc.identifier.affiliationsDepartment of Biotechnology, Institute of Genomics and Integrative Biology,Council of Scientific and Industrial Research, New Delhi, India.en_US
dc.identifier.citationAgarwal Nikki, Kapoor Seema, Mangla Ankit, Kumar Ashok, Mandal Ravi N, Kumar Namita Ravi, Ramachandran Mohanaraj. Utility of Detecting sof Gene as Evidence of Streptococcus pyogenes Infection in Acute Rheumatic Fever . Indian Pediatrics. 2019 Apr; 56(4): 311-313en_US
dc.identifier.issn0974-7559
dc.identifier.issn0019-6061
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/199310
dc.languageenen_US
dc.publisherIndian Academy of Pediatricsen_US
dc.relation.issuenumber4en_US
dc.relation.volume56en_US
dc.source.urihttps://www.indianpediatrics.net/apr2019/311.pdfen_US
dc.subjectDiagnosisen_US
dc.subjectRheumatic heart diseaseen_US
dc.subjectStreptococcal infectionen_US
dc.titleUtility of Detecting sof Gene as Evidence of Streptococcus pyogenes Infection in Acute Rheumatic Feveren_US
dc.typeJournal Articleen_US
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