In-silico analysis of Porcine and recombinant Insulin activity on Glycogen
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Date
2020-05
Journal Title
Journal ISSN
Volume Title
Publisher
Medical Council of India
Abstract
The study focuses on the anti-diabetic activity by molecular simulation of Recombinant Insulin, PorcineInsulin, and Glycogen. The sequence of these three molecules was retrieved, and 3D structures weremodeled. A total of two different molecular simulations were carried out. The simulations were done usingAutodock software. Initially, the downloaded PDB structures were docked with glycogen and the secondbetween the active site peptide models of both insulin molecules based on castP prediction with glycogenmolecule. The results were analyzed by Ramachandran plot for model prediction, and the binding energywas set as criteria to determine the best-docked model. The binding energy of recombinant insulin, porcineinsulin with glycogen was 0.32 and -1.09 respectively. Similarly, the binding energy for peptide modelswith glycogen molecule was found to be +1.09 and +6.76 respectively. Based on the results, it wasconcluded that the recombinant insulin has higher affinity than the porcine insulin.
Description
Keywords
Recombinant Insulin, Porcine Insulin, Glycogen, 7INS, 3i40, Molecular simulations, Active site prediction
Citation
Ezra Leander Santhosh, Irudhayaraj Navinraj Theneri, Elango Kishor, Irudhayaraj Julie Selvi. In-silico analysis of Porcine and recombinant Insulin activity on Glycogen. International Journal of Interdisciplinary and Multidisciplinary Studies. 2020 May; 7(2): 52-64