Effect of T3 treatment on glutathione redox pool and its metabolizing enzymes in mitochondrial and post-mitochondrial fractions of adult rat testes.

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dc.contributor.authorSahoo, Dipak Ken_US
dc.contributor.authorRoy, Anitaen_US
dc.contributor.authorChattopadhyay, Sutapaen_US
dc.contributor.authorChainy, G B Nen_US
dc.date.accessioned2009-05-28T11:32:50Z
dc.date.available2009-05-28T11:32:50Z
dc.date.issued2007-04-05en_US
dc.description.abstractT3 (3,3', 5-triiodo-L-thyronine; 20 microg/100 g body weight/day in 0.01 N NaOH, i.p for 1, 3 and 5 days) treatment modulated reduced (GSH) and oxidized (GSSG) glutathione contents along with the activities of its metabolizing enzymes (such as glutathione peroxidase, glutathione reductase and glutathione S-transferase) in the testis of Wistar rats. However, the magnitude and nature of changes in the above biochemical parameters in response to T3 treatment were noticed to be different between mitochondrial and post-mitochondrial fractions. This was accompanied with elevated levels of lipid hydroperoxide and ascorbic acid in the crude homogenate of testis. The level of hydrogen peroxide in the post-mitochondrial fractions of testes did not change on first day, decreased on 3rd day and increased on 5th day of the hormone treatment when compared to respective controls. Nevertheless, its content in mitochondria was significantly elevated in response to all the three durations of the hormone treatment having the highest induction on 3rd day. The changes observed in the levels of GSH and GSSG and its metabolizing enzymes in response to T3 treatment reflect an alteration in the redox state of testis, which may be a causative factor for the impairment of testicular physiology as a consequence of oxidative stress.en_US
dc.description.affiliationDepartment of Zoology, Utkal University, Bhubaneswar 751 004, India.en_US
dc.identifier.citationSahoo DK, Roy A, Chattopadhyay S, Chainy GB. Effect of T3 treatment on glutathione redox pool and its metabolizing enzymes in mitochondrial and post-mitochondrial fractions of adult rat testes. Indian Journal of Experimental Biology. 2007 Apr; 45(4): 338-46en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56866
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/5259/1/IJEB%2045%284%29%20338-346.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Fractionationen_US
dc.subject.meshGlutathione --metabolismen_US
dc.subject.meshGlutathione Disulfide --analysisen_US
dc.subject.meshGlutathione Peroxidase --metabolismen_US
dc.subject.meshGlutathione Reductase --metabolismen_US
dc.subject.meshGlutathione Transferase --metabolismen_US
dc.subject.meshHydrogen Peroxide --metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMitochondria --enzymologyen_US
dc.subject.meshOxidation-Reductionen_US
dc.subject.meshOxidative Stressen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshTestis --drug effectsen_US
dc.subject.meshTriiodothyronine --pharmacologyen_US
dc.titleEffect of T3 treatment on glutathione redox pool and its metabolizing enzymes in mitochondrial and post-mitochondrial fractions of adult rat testes.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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