Qualitative and Quantitative Analysis of Phylloplane Mycoflora of Stevia Rebaudiana Bertoni
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Date
2019-01
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SB Publications
Abstract
The aim of the present investigation is to study the fungal flora of young and matureleaves of S. rebaudiana. Young and mature leaves of S. rebaudiana were used for isolation offungi. Phylloplane mycoflora were isolated from leaves of S. rebaudiana by using threemethods i.e. dilution plate method, leaf impression method and leaf washing method. Dilutionplate method and leaf washing method were applied for quantitative analysis and leafimpression method for qualitative analysis. A total of 35 fungal species comprisingZygomycetes, Ascomycetes and Deuteromycetes including sterile forms were isolated duringthe course of investigation. A total of thirteen fungal species viz. Aspergillus niger, A. flavus, A.fumigatus, Penicillium cyclopium, P. chrysogenum, Cladosporium elatum, C. cladosporioides,Mucor etc. were isolated from mature leaves. Eleven fungal species were found by leafimpression method, eight by leaf washing method and six by dilution plate method.Aspergillus, Penicillium and Cladosporium species were found to be dominant among all thespecies. Phylloplane fungal population on mature leaves of S. rebaudiana was 1.68×102 /cm2leaf area. Thus, the outcome of study presents a clear picture about fungal population anddiversity in phylloplane of S. rebaudiana mature leaves.
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Keywords
Dilution plate, Stevia rebaudiana;Leaf washing, Leaf impression, Fungi
Citation
Chauhan Reshu, Navneet. Qualitative and Quantitative Analysis of Phylloplane Mycoflora of Stevia Rebaudiana Bertoni. Journal of Biological Engineering Research and Review. 2019 Jan; 6(1): 1-7