Presence of extracellular DNA & protein in biofilm formation by gentamicin-resistant Lactobacillus plantarum

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dc.contributor.authorGeorge, Jen_US
dc.contributor.authorHalami, PMen_US
dc.date.accessioned2020-04-10T01:41:46Z
dc.date.available2020-04-10T01:41:46Z
dc.date.issued2019-02
dc.description.abstractBackground & objectives: Bacterial biofilms a multi-layered defence, comprise extracellular DNA (eDNA) and proteins, protect bacteria from harmful environment and nutrient limitation and utilize the mutual benefits within a community. Bacterial biofilms also defend bacteria from harsh environments such as antibiotic treatment. This leads to poor antibiotic penetration, slow growth, adaptive stress responses, and formation of persister cells. This study was done to determine the relation of antibiotic resistance deciphered by the biofilms in Lactobacillus plantarum, a lactic acid bacteria (LAB) with probiotic significance. Methods: The gentamicin-resistant L. plantarum isolates were allowed to form biofilms and subjected to DNase I and proteinase K treatment. The optical density (OD) values were recorded for the biofilm assay and the cell count for the number of viable cells was taken for the control and the test samples. Percentage reduction was calculated based on the difference between the initial and final OD for both the parameters. Results: The biofilm assay revealed that the native L. plantarum isolates which were phenotypically susceptible, possessed the ability to form biofilms. The OD values were significantly decreased in comparison to the biofilm-forming control culture when these were treated with DNase I and proteinase K. Interpretation & conclusions: The study revealed that the biofilms formed by L. plantarum comprised of eDNA and proteins which was evidenced by the reduction in OD values and percentage in comparison to the control upon DNase I and proteinase K treatment. This indicates that the eDNA and biofilm matrix proteins are vital constituents of biofilms and may carry significant risk when coupled with antibiotic resistance.en_US
dc.identifier.affiliationsDepartment of Microbiology & Fermentation Technology, CSIR-Central Food Technological Research Institute, Mysuru, Indiaen_US
dc.identifier.citationGeorge J, Halami PM. Presence of extracellular DNA & protein in biofilm formation by gentamicin-resistant Lactobacillus plantarum. Indian Journal of Medical Research. 2019 Feb; 149(2): 257-262en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/195802
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber2en_US
dc.relation.volume149en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_2022_17en_US
dc.subjectAntibiotic resistanceen_US
dc.subjectbiofilmsen_US
dc.subjectextracellular DNAen_US
dc.subjectlactic acid bacteriaen_US
dc.subjectnutrient limitationen_US
dc.subjectpersister cellsen_US
dc.titlePresence of extracellular DNA & protein in biofilm formation by gentamicin-resistant Lactobacillus plantarumen_US
dc.typeJournal Articleen_US
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