Biofilm Formation and Antifungal Susceptibility of Candida Isolates from Various Clinical Specimens.

Simple item page
dc.contributor.authorMohamed, Sahar Ali
dc.contributor.authorAl-Ahmadey, Ziab Zakey
dc.date.accessioned2015-09-02T11:38:39Z
dc.date.available2015-09-02T11:38:39Z
dc.date.issued2013-10
dc.description.abstractBackground: Candida species are now recognized as major causative agents of hospitalacquired infection. Aims: To evaluate the species distribution, biofilm formation,and antifungal susceptibility (amphotericin B, ketoconazole, and fluconazole) of Candida isolates. Place and Duration of Study: This is a Six-months Cross sectional study conducted in Alansar hospital, Al-Madinah, Saudi Arabia. Methodology: One hundred and three isolates of Candida spp. were cultured on Sabouraud dextrose agar (SDA). Candida spp. were identified by four standard methods, CHROMagar candida, cornmeal agar, germ tube test and API 20C. Detection of Biofilm formation was done by microtitre plate and antifungal susceptibility testing was done by disc diffusion. Results: C. albicans was the most common species 61%, followed by C. tropicalis 25%, C. lusitanaie 5%, C. parapsilosis 4%, C. glabrata 4% and C. famata 1%. Biofilm formation was found to occur most frequently among non-albicans spp.(70%) than C. albicans (46%). All isolates were sensitive to amphotericin B and ketoconazole. Resistance to fluconazole was found in 22.5% of non-albicans spp. and 5% of C. albicans isolates. Conclusion: The present study proved that C. albicans is still the major isolate from urinary, vaginal and respiratory samples but non-albicans spp. predominate in the blood samples and from plastic devices. The non-albicans spp. were more biofilm - producers compared to C. albicans and C. tropicalis showed the highest score of biofilm intensity (grade 4+). The species isolated are less susceptible to fluconazole.en_US
dc.identifier.citationMohamed Sahar Ali, Al-Ahmadey Ziab Zakey. Biofilm Formation and Antifungal Susceptibility of Candida Isolates from Various Clinical Specimens. British Microbiology Research Journal. 2013 Oct; 3(4): 590-601.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/162946
dc.language.isoenen_US
dc.source.urihttps://sciencedomain.org/abstract/1914en_US
dc.subjectCandida spp.en_US
dc.subjectnon-albicans spp.en_US
dc.subjectbiofilm formationen_US
dc.subjectantifungalen_US
dc.subject.meshAntifungal Agents --pharmacology
dc.subject.meshBiofilms --growth & development
dc.subject.meshBiofilms --physiology
dc.subject.meshCandida --classification
dc.subject.meshCandida --metabolism
dc.subject.meshCandida albicans --metabolism
dc.subject.meshCross-Sectional Studies
dc.subject.meshHumams
dc.subject.meshRespiratory System --microbiology
dc.subject.meshUrinary Tract --microbiology
dc.subject.meshVagina --microbiology
dc.titleBiofilm Formation and Antifungal Susceptibility of Candida Isolates from Various Clinical Specimens.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
bmrj2013v3n4p590.pdf
Size:
1.78 MB
Format:
Adobe Portable Document Format
Description:
Original research article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Microbiology Research Journal International