Occurrence of Cucumber mosaic virus on vanilla (Vanilla planifolia Andrews) in India.

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dc.contributor.authorMadhubala, Ren_US
dc.contributor.authorBhadramurthy, Ven_US
dc.contributor.authorBhat, A Ien_US
dc.contributor.authorHareesh, P Sen_US
dc.contributor.authorRetheesh, S Ten_US
dc.contributor.authorBhai, R Sen_US
dc.date.accessioned2005-06-30en_US
dc.date.accessioned2009-06-01T15:04:07Z
dc.date.available2005-06-30en_US
dc.date.available2009-06-01T15:04:07Z
dc.date.issued2005-06-30en_US
dc.description.abstractCucumber mosaic virus (CMV) causing mosaic, leaf distortion and stunting of vanilla (Vanilla planifolia Andrews) in India was characterized on the basis of biological and coat protein (CP) nucleotide sequence properties. In mechanical inoculation tests, the virus was found to infect members of Chenopodiaceae, Cucurbitaceae, Fabaceae and Solanaceae. Nicotiana benthamiana was found to be a suitable host for the propagation of CMV. The virus was purified from inoculated N. benthamiana plants and negatively stained purified preparations contained isometric particles of about 28 nm in diameter. The molecular weight of the viral coat protein subunits was found to be 25.0 kDa. Polyclonal antiserum was produced in New Zealand white rabbit, immunoglobulin G (IgG) was purified and conjugated with alkaline phosphatase enzyme. Double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) method was standardized for the detection of CMV infection in vanilla plants. CP gene of the virus was amplified using reverse transcriptase-polymerase chain reaction (RT-PCR), cloned and sequenced. Sequenced region contained a single open reading frame of 657 nucleotides potentially coding for 218 amino acids. Sequence analyses with other CMV isolates revealed the greatest identity with black pepper isolate of CMV (99%) and the phylogram clearly showed that CMV infecting vanilla belongs to subgroup IB. This is the first report of occurrence of CMV on V. planifolia from India.en_US
dc.description.affiliationDivision of Crop Protection, Indian Institute of Spices Research, Calicut 673 012, India.en_US
dc.identifier.citationMadhubala R, Bhadramurthy V, Bhat AI, Hareesh PS, Retheesh ST, Bhai RS. Occurrence of Cucumber mosaic virus on vanilla (Vanilla planifolia Andrews) in India. Journal of Biosciences. 2005 Jun; 30(3): 339-50en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111279
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshCapsid Proteins --chemistryen_US
dc.subject.meshCucumovirus --geneticsen_US
dc.subject.meshIndiaen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshPlant Diseases --virologyen_US
dc.subject.meshPlant Leaves --physiologyen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshVanilla --virologyen_US
dc.titleOccurrence of Cucumber mosaic virus on vanilla (Vanilla planifolia Andrews) in India.en_US
dc.typeJournal Articleen_US
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