Dynamic expression pattern of kinesin accessory protein in Drosophila.

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dc.contributor.authorSarpal, Rituen_US
dc.contributor.authorRay, Krishanuen_US
dc.date.accessioned2002-09-17en_US
dc.date.accessioned2009-06-01T14:12:47Z
dc.date.available2002-09-17en_US
dc.date.available2009-06-01T14:12:47Z
dc.date.issued2002-09-17en_US
dc.description.abstractWe have identified the Drosophila homologue of the non-motor accessory subunit of kinesin-II motor complex. It is homologous to the SpKAP115 of the sea urchin, KAP3A and KAP3B of the mouse, and SMAP protein in humans. In situ hybridization using a DmKAP specific cRNA probe has revealed a dynamic pattern of expression in the developing nervous system. The staining first appears in a subset of cells in the embryonic central nervous system at stage 13 and continues till the first instar larva stage. At the third instar larva stage the staining gets restricted to a few cells in the optic lobe and in the ventral ganglion region. It has also stained a subset of sensory neurons from late stage 13 and till the first instar larva stage. The DmKAP expression pattern in the nervous system corresponds well with that of Klp64D and Klp68D as reported earlier. In addition, we have found that the DmKAP gene is constitutively expressed in the germline cells and in follicle cells during oogenesis. These cells are also stained using an antibody to KLP68D protein, but mRNA in situ hybridization using KLP64D specific probe has not stained these cells. Together these results proved a basis for further analysis of tissue specific function of DmKAP in future.en_US
dc.description.affiliationDepartment of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India.en_US
dc.identifier.citationSarpal R, Ray K. Dynamic expression pattern of kinesin accessory protein in Drosophila. Journal of Biosciences. 2002 Sep; 27(5): 479-87en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/110668
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCentral Nervous System --metabolismen_US
dc.subject.meshDrosophila --embryologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshKinesin --geneticsen_US
dc.subject.meshLarva --metabolismen_US
dc.subject.meshRNA Probesen_US
dc.subject.meshRNA, Messenger --geneticsen_US
dc.titleDynamic expression pattern of kinesin accessory protein in Drosophila.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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