WNIN/GR-Ob - An insulin-resistant obese rat model from inbred WNIN strain.

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dc.contributor.authorHarishankar, N
dc.contributor.authorVajreswari, A
dc.contributor.authorGiridharan, N V
dc.date.accessioned2011-12-08T09:36:48Z
dc.date.available2011-12-08T09:36:48Z
dc.date.issued2011-09
dc.description.abstractBackground & objectives: WNIN/GR-Ob is a mutant obese rat strain with impaired glucose tolerance (IGT) developed at the National Institute of Nutrition (NIN), Hyderabad, India, from the existing 80 year old Wistar rat (WNIN) stock colony. The data presented here pertain to its obese nature along with IGT trait as evidenced by physical, physiological and biochemical parameters. The study also explains its existence, in three phenotypes: homozygous lean (+/+), heterozygous carrier (+/-) and homozygous obese (-/-). Methods: Thirty animals (15 males and 15 females) from each phenotype (+/+, +/-, -/-) and 24 lean and obese (6 males and 6 females) rats were taken for growth and food intake studies respectively. Twelve adult rats from each phenotype were taken for body composition measurement by total body electrical conductivity (TOBEC); 12 rats of both genders from each phenotype at different ages were taken for clinical chemistry parameters. Physiological indices of insulin resistance were calculated according to the homeostasis model assessment for insulin resistance (HOMA-IR) and also by studying U14C 2-deoxy glucose uptake (2DG). Results: WNINGR-Ob mutants had high growth, hyperphagia, polydipsia, polyurea, glycosuria, and significantly lower lean body mass, higher fat mass as compared with carrier and lean rats. These mutants, at 50 days of age displayed abnormal response to glucose load (IGT), hyperinsulinaemia, hypertriglyceridaemia, hypercholesterolaemia and hyperleptinaemia. Basal and insulin-stimulated glucose uptakes by diaphragm were significantly decreased in obese rats as compared with lean rats. Interpretation & conclusions: Obese rats of the designated WNIN/GR-Ob strain showed obesity with IGT, as adjudged by physical, physiological and biochemical indices. These indices varied among the three phenotypes, being lowest in lean, highest in obese and intermediate in carrier phenotypes thereby suggesting that obesity is inherited as autosomal incomplete dominant trait in this strain. This mutant obese rat model is easy to propagate, and can easily be transformed to frank diabetes model by dietary manipulation and thus can be used for screening anti-diabetic drugs.en_US
dc.identifier.citationHarishankar N, Vajreswari A, Giridharan N V. WNIN/GR-Ob - An insulin-resistant obese rat model from inbred WNIN strain. Indian Journal of Medical Research. 2011 Sept; 134(3): 320-329.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135764
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193713/en_US
dc.subjectHyperinsulinaemiaen_US
dc.subjecthyperleptinaemiaen_US
dc.subjectimpaired glucose toleranceen_US
dc.subjectlean body massen_US
dc.subjectTOBECen_US
dc.subject.meshAnimals
dc.subject.meshBlood Glucose
dc.subject.meshBody Composition
dc.subject.meshBreeding --methods
dc.subject.meshDisease Models, Animal
dc.subject.meshElectric Conductivity
dc.subject.meshFemale
dc.subject.meshIndia
dc.subject.meshInsulin --blood
dc.subject.meshInsulin Resistance --genetics
dc.subject.meshMale
dc.subject.meshObesity --genetics
dc.subject.meshPhenotype
dc.subject.meshRats
dc.subject.meshRats, Wistar
dc.titleWNIN/GR-Ob - An insulin-resistant obese rat model from inbred WNIN strain.en_US
dc.typeArticleen_US
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