Effects of Russell's viper venom on mediator production in cultured human umbilical vein endothelial cells.

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dc.contributor.authorThamaree, Sen_US
dc.contributor.authorSitprija, Ven_US
dc.contributor.authorPunyavoravuth, Ven_US
dc.contributor.authorAkarasereenont, Pen_US
dc.contributor.authorPuckmanee, Nen_US
dc.contributor.authorKhow, Oen_US
dc.contributor.authorThaworn, Nen_US
dc.date.accessioned2009-05-27T19:04:19Z
dc.date.available2009-05-27T19:04:19Z
dc.date.issued2001-06-01en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractHemodynamic alterations in Russell's viper envenomation are the result of interactions of various vasoactive mediators and perhaps proinflammatory cytokines. Since vascular endothelium is likely to be exposed to high concentrations of the venom and the endothelial cell itself not only plays an important role in the physiologic control of the circulation, but also play a role in inflammation with the synthesis and secretion of proinflammatory cytokines. It was therefore, the objective of this study to determine the effects of Russell's viper venom (RVV) on proinflammatory cytokine production by cultured human umbilical vein endothelial cells (HUVEC) and the release of endothelium-derived substances. Endothelial cells were isolated from freshly obtained human umbilical cord vein and grown in tissue culture to confluence as a homogeneous population. Cells were then incubated at 37 degrees C under 5 per cent CO2 with RVV (0.2, 1.0, 5.0, and 25.0 microg/ml) or lipopolysaccharide (LPS, 10 microg/ml) for 3, 6, 12 and 24 hours. After an indicated time, the levels of endothelin-1 (ET-1); 6-keto-PGF1alpha (a stable metabolite of PGI2) tumor necrosis factor-alpha (TNF-alpha); interleukin-1beta (IL-1beta); and interleukin-6 (IL-6) in supernatants were measured by using ELISA or EIA. The effect of RVV or LPS on cell viability was also measured using MIT assay. The results showed copious amounts of ET-1 production irrespectively with the presence of RVV or LPS. Whereas, production of PGI2 (measured as 6-keto-PGF1alpha, a stable metabolite) was increased significantly higher in the RVV- and LPS-treated EC than in the control EC. However, TNF-alpha and IL-6 productions were not different among these groups. The levels of IL-1beta were very low, although IL-1beta was detectable in the group treated with RVV at a concentration of 25.0 microg/ml. In conclusion, RVV upto 25 microg/ml stimulated PGI2 production by cultured HUVEC. This effect was unlikely related to production of proinflammatory cytokines since LPS or RVV is not sufficient per se to elevate a substantial amount of EC-derived cytokines. The higher amount of IL-6 compared to TNF-alpha and IL-1beta may be produced through other pathways apart from production via a cascade of cytokines. This is the first report showing that RVV up to 25 microg/ml has no effect on prominent proinflammatory cytokine production by HUVEC. However, in blood circulation, the major source of cytokines production is monocyte-macrophage lineage cell. Thus, RVV in blood circulation may activate the production of proinflammatory cytokines mainly from those cells and subsequently induce toxicity.en_US
dc.description.affiliationDepartment of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.en_US
dc.identifier.citationThamaree S, Sitprija V, Punyavoravuth V, Akarasereenont P, Puckmanee N, Khow O, Thaworn N. Effects of Russell's viper venom on mediator production in cultured human umbilical vein endothelial cells. Journal of the Medical Association of Thailand. 2001 Jun; 84 Suppl 1(): S197-207en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/40413
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAnalysis of Varianceen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCytokines --biosynthesisen_US
dc.subject.meshEndothelium, Vascular --cytologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInflammation Mediators --analysisen_US
dc.subject.meshProbabilityen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshUmbilical Veins --cytologyen_US
dc.subject.meshViper Venoms --pharmacologyen_US
dc.titleEffects of Russell's viper venom on mediator production in cultured human umbilical vein endothelial cells.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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