Phenylpropanoid enzymes, phenolic polymers and metabolites as chemical defenses to infection of Pratylenchus coffeae in roots of resistant and susceptible bananas (Musa spp.).

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dc.contributor.authorVaganan, M Mayil
dc.contributor.authorRavi, I
dc.contributor.authorNandakumar, A
dc.contributor.authorSarumathi, S
dc.contributor.authorSundararaju, P
dc.contributor.authorMustaffa, M M
dc.date.accessioned2014-04-30T04:53:07Z
dc.date.available2014-04-30T04:53:07Z
dc.date.issued2014-03
dc.description.abstractActivity differences of the first (phenylalanine ammonia lyase, PAL) and the last (cinnamyl alcohol dehydrogenase, CAD) enzymes of phenylpropanoid pathway in the roots of resistant (Yangambi Km5 and Anaikomban) and susceptible (Nendran and Robusta) banana cultivars caused by root lesion nematode, Pratylenchus coffeae, were investigated. Also, the accumulation of phenolics and deposition of lignin polymers in cell walls in relation to resistance of the banana cultivars to the nematode were analyzed. Compared to the susceptible cultivars, the resistant cultivars had constitutively significantly higher PAL activity and total soluble and cell wall-bound phenolics than in susceptible cultivars. The resistant cultivars responded strongly to the infection of the nematode by induction of several-time higher PAL and CAD enzymes activities, soluble and wall-bound phenolics and enrichment of lignin polymers in cell wall and these biochemical parameters reached maximum at 7th day postinoculation. In addition, profiles of phenolic acid metabolites in roots of Yangambi Km5 and Nendran were analyzed by HPLC to ascertain the underlying biochemical mechanism of bananas resistance to the nematode. Identification and quantification of soluble and cell wall-bound phenolic acids showed six metabolites and only quantitative, no qualitative, differences occurred between the resistant and susceptible cvs. and between constitutive and induced contents. A very prominent increase of p-coumaric, ferulic and sinapic acids, which are precursors of monolignols of lignin, in resistant cv. was found. These constitutive and induced biochemical alterations are definitely the chemical defenses of resistant cvs. to the nematode infection.en_US
dc.identifier.citationVaganan M Mayil, Ravi I, Nandakumar A, Sarumathi S, Sundararaju P, Mustaffa M M. Phenylpropanoid enzymes, phenolic polymers and metabolites as chemical defenses to infection of Pratylenchus coffeae in roots of resistant and susceptible bananas (Musa spp.). Indian Journal of Experimental Biology. 2014 Mar; 52(3): 252-260.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/150355
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/27349en_US
dc.subjectCinnamyl alcohol dehydrogenaseen_US
dc.subjectp-Coumaric aciden_US
dc.subjectFerulic aciden_US
dc.subjectLigninen_US
dc.subjectPhenylalanine ammonia lyaseen_US
dc.subjectRoot lesion nematodeen_US
dc.subjectYangambi Km5en_US
dc.subject.meshAlcohol Oxidoreductases --genetics
dc.subject.meshAlcohol Oxidoreductases --metabolism
dc.subject.meshAnimals
dc.subject.meshDisease Resistance --genetics
dc.subject.meshMetabolic Networks and Pathways
dc.subject.meshMusa --enzymology
dc.subject.meshMusa --genetics
dc.subject.meshMusa --growth & development
dc.subject.meshMusa --parasitology
dc.subject.meshNematoda --genetics
dc.subject.meshNematoda --pathogenicity
dc.subject.meshPhenols --chemistry
dc.subject.meshPhenols --metabolism
dc.subject.meshPhenylalanine Ammonia-Lyase --genetics
dc.subject.meshPhenylalanine Ammonia-Lyase --metabolism
dc.subject.meshPlant Diseases --genetics
dc.subject.meshPlant Diseases --parasitology
dc.subject.meshPlant Roots --enzymology
dc.subject.meshPlant Roots --metabolism
dc.subject.meshPolymers --chemistry
dc.subject.meshPropanols --chemistry
dc.subject.meshPropanols --metabolism
dc.titlePhenylpropanoid enzymes, phenolic polymers and metabolites as chemical defenses to infection of Pratylenchus coffeae in roots of resistant and susceptible bananas (Musa spp.).en_US
dc.typeArticleen_US
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