Extracellular amylase(s) production by fungi Botryodiplodia theobromae and Rhizopus oryzae grown on cassava starch residue.

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dc.contributor.authorRay, R Cen_US
dc.date.accessioned2004-10-24en_US
dc.date.accessioned2009-06-02T04:06:15Z
dc.date.available2004-10-24en_US
dc.date.available2009-06-02T04:06:15Z
dc.date.issued2004-10-24en_US
dc.description.abstractThe fungi Botryodiplodia theobromae and Rhizopus oryzae produce extracellular amylase when grown on a liquid medium containing 2% (WN) soluble starch or cassava starch residue(CSR) (as starch equivalent), a waste generated after extraction of starch from cassava, as the sole carbon source. Using CSR as the sole carbon source, the highest amylase activity of 3.25 and 3.8 units (mg, glucose released x ml(-1) x h(-1)) were obtained in shake flask cultures during the late stationary phase of growth of B. theobromae and R. oryzae, respectively. These values were slightly lower than the values obtained using soluble starch as the carbon source. Maximum enzyme synthesis in CSR incorporated medium occurred at the growth temperature of 30 degrees C and pH 6.0. Presence of inorganic NH4+ salts like ammonium acetate and ammonium nitrate in culture medium yielded more amylase than the other nitrogen sources. Amylase(s) production in the controlled environment of a Table-Top glass Jar Fermenter (2-L capacity) was 4.8 and 5.1 units for B. theobromae and R. oryzae, respectively using CSR as the carbon substrate. It is concluded that CSR, a cheap agricultural waste obtained after starch extraction from cassava could replace soluble starch as carbon substrate for commercial production of fungal amylase(s).en_US
dc.description.affiliationCentral Tuber Crops Research Institute (Regional Center), P.O. Dumduma, Bhubaneswar-751 019, India. rc_ray@rediffmail.comen_US
dc.identifier.citationRay RC. Extracellular amylase(s) production by fungi Botryodiplodia theobromae and Rhizopus oryzae grown on cassava starch residue. Journal of Environmental Biology. 2004 Oct; 25(4): 489-95en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/113535
dc.language.isoengen_US
dc.source.urihttps://www.geocities.com/j_environ_biol/en_US
dc.subject.meshAmylases --metabolismen_US
dc.subject.meshAscomycota --metabolismen_US
dc.subject.meshBioreactorsen_US
dc.subject.meshCarbon --metabolismen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshManihoten_US
dc.subject.meshNitrogen --metabolismen_US
dc.subject.meshRhizopus --metabolismen_US
dc.subject.meshStarchen_US
dc.subject.meshTemperatureen_US
dc.titleExtracellular amylase(s) production by fungi Botryodiplodia theobromae and Rhizopus oryzae grown on cassava starch residue.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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