Evaluation of biomarkers: Galactomannan and 1,3-beta-D-glucan assay for the diagnosis of invasive fungal infections in immunocompromised patients from a tertiary care centre

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dc.contributor.authorSingh, Sen_US
dc.contributor.authorKaur, Hen_US
dc.contributor.authorChoudhary, Hen_US
dc.contributor.authorSethi, Sen_US
dc.contributor.authorMalhotra, Pen_US
dc.contributor.authorGupta, KLen_US
dc.contributor.authorRudramurthy, SMen_US
dc.contributor.authorChakrabarti, Aen_US
dc.date.accessioned2020-04-23T07:37:17Z
dc.date.available2020-04-23T07:37:17Z
dc.date.issued2018-12
dc.description.abstractPurpose: Due to limitations of traditional microbiological techniques, standardised fungal biomarker tests such as Galactomannan Index (GMI) and 1,3-beta-D-glucan (BDG) are being preferred for diagnosis of invasive fungal infections (IFIs). These tests have been extensively used in developed countries but seldom in developing countries. The present study was performed to evaluate these tests for the diagnosis of IFIs in immunocompromised patients at an Indian tertiary care centre. Materials and Methods: A retrospective hospital-based study was done in immunocompromised patients with clinical suspicion of IFI. The demographic, clinical, radiological and mycological details of the patients were recorded. The patients were categorised into proven, probable and no IFI (as per European Organization for Research and Treatment of Cancer/Mycoses Study Group criteria). The sensitivity and specificity of BDG Fungitell and Platelia Aspergillus antigen assays was estimated. Results: A total of 70 consecutive patients were included, of which 41 had IFI (10 proven and 31 probable) while 29 had no IFI. A significant association was found between IFI and the presence of a central venous line (P = 0.035) and history of intake of T-cell immunosuppressants (P = 0.001). Median BDG values (pg/ml) in patients with proven IFI, probable IFI and no IFI were 300 (range: 70�0), 165 (range: 53�0) and 45 (range: 31�0), respectively. The receiver operating characteristic (ROC) curve analysis for BDG revealed an area under the curve of 0.995, sensitivity: 97.4% and specificity: 96.6% for IFI diagnosis. The ROC curve analysis of GMI revealed an AUC of 0.75 and 90% patients with invasive aspergillosis (IA) had positive GMI. Conclusion: BDG has good sensitivity and specificity for distinguishing IFI from no IFIs and GMI may be used for diagnosing IA.en_US
dc.identifier.affiliationsDepartment of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, Indiaen_US
dc.identifier.affiliationsDepartment of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, Indiaen_US
dc.identifier.affiliationsDepartment of Nephrology, Postgraduate Institute of Medical Education and Research, Chandigarh, Indiaen_US
dc.identifier.citationSingh S, Kaur H, Choudhary H, Sethi S, Malhotra P, Gupta KL, Rudramurthy SM, Chakrabarti A. Evaluation of biomarkers: Galactomannan and 1,3-beta-D-glucan assay for the diagnosis of invasive fungal infections in immunocompromised patients from a tertiary care centre. Indian Journal of Medical Microbiology. 2018 Dec; 36(4): 557-563en_US
dc.identifier.issn0255-0857
dc.identifier.issn1998-3646
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/198817
dc.languageenen_US
dc.publisherIndian Association of Medical Microbiologistsen_US
dc.relation.issuenumber4en_US
dc.relation.volume36en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmm.IJMM_18_366en_US
dc.subjectBeta-D-glucanen_US
dc.subjectgalactomannanen_US
dc.subjectinvasive fungal infectionen_US
dc.subjectimmunocompromiseden_US
dc.subjectIndiaen_US
dc.titleEvaluation of biomarkers: Galactomannan and 1,3-beta-D-glucan assay for the diagnosis of invasive fungal infections in immunocompromised patients from a tertiary care centreen_US
dc.typeJournal Articleen_US
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