Antimicrobial activity of flavanoid sulphates and other fractions of Argyreia speciosa (Burm.f) Boj.

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dc.contributor.authorHabbu, P Ven_US
dc.contributor.authorMahadevan, K Men_US
dc.contributor.authorShastry, R Aen_US
dc.contributor.authorManjunatha, Hen_US
dc.date.accessioned2009-05-28T14:50:40Z
dc.date.available2009-05-28T14:50:40Z
dc.date.issued2009-02-21en_US
dc.description.abstractAntimicrobial activity of flavanoid sulphates and different fractions of A. speciosa root was studied against bacteria, fungi and Mycobacterium tuberculosis H37 Rv sensitive strain by in vitro and in vivo assays. Flavanoid sulphates such as quercetin 3'7 di-O methyl 3- sulphate and kaempferol 7-O methyl 3-sulphate were isolated from the n-butanol fraction of 80% methanolic extract of the plant. The structures of the isolated flavanoids were confirmed by spectral studies. Ethyl acetate (EAAS) fraction and flavanoid sulphates inhibited the growth of M. tuberculosis Rv sensitive strain at MIC values 50 and 25 microg/ml, respectively. Ethanolic fraction (EtAS) showed significant inhibition of gram positive organism with a MIC of 31.25 microg/ml. More inhibition was observed with a less MIC (2 microg/ml) for flavanoid sulphates against Klebsiella pneumoniae, a gram negative organism and it is almost comparable with the standards. Interestingly, chloroform fraction alone exhibited significant antifungal activity with a MIC of 100 microg/ml. A synergistic effect between flavanoids sulphates and commercially available antitubercular drugs was observed with FIC index of 0.443 +/- 0.245, 0.487 +/- 0.247 for isoniazid and 0.468 +/- 0.333, 0.417 +/- 0.345 for rifampicin, whereas EAAS fraction showed partial synergistic effect. A synergistic effect was observed for EAAS fraction and flavanoids sulphates with FIC index < 0.5 with antibiotics. Hemolysis assay on RBCs suggested that EAAS and flavanoids sulphates exhibited least cellular toxicity to erythrocytes as compared to chloramphenicol. In vivo studies in mice infected with K. pneumoniae demonstrated that on day 10 post treatment of different fractions and isolated compounds of A. speciosa, about 60% of the animals treated with EAAS, 70% of animals treated with flavanoids sulphates and 40% of animals treated with EtAS were survived.en_US
dc.description.affiliationPostgraduate Department, Division of Pharmacognosy, SET's College of Pharmacy, Dharwad 580 002, India. prasherbs@yahoo.comen_US
dc.identifier.citationHabbu PV, Mahadevan KM, Shastry RA, Manjunatha H. Antimicrobial activity of flavanoid sulphates and other fractions of Argyreia speciosa (Burm.f) Boj. Indian Journal of Experimental Biology. 2009 Feb; 47(2): 121-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/61229
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/3074/1/IJEB%2047%282%29%20121-128.pdfen_US
dc.subject.meshAnimals
dc.subject.meshAnti-Bacterial Agents --pharmacology
dc.subject.meshBacteria --drug effects
dc.subject.meshCell Survival --drug effects
dc.subject.meshConvolvulaceae --chemistry
dc.subject.meshErythrocytes --cytology
dc.subject.meshErythrocytes --drug effects
dc.subject.meshFlavonoids --chemistry
dc.subject.meshFlavonoids --pharmacology
dc.subject.meshFungi --drug effects
dc.subject.meshHumans
dc.subject.meshMedicine, Ayurvedic
dc.subject.meshMice
dc.subject.meshMicrobial Viability --drug effects
dc.subject.meshPlant Extracts --pharmacology
dc.subject.meshPlant Roots --chemistry
dc.subject.meshSulfates --chemistry
dc.titleAntimicrobial activity of flavanoid sulphates and other fractions of Argyreia speciosa (Burm.f) Boj.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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