Crystal violet stain as a selective stain for the assessment of mitotic figures in oral epithelial dysplasia and oral squamous cell carcinoma.

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dc.contributor.authorJadhav, Kiran B
dc.contributor.authorMujib, B R Ahmed
dc.contributor.authorGupta, Nidhi
dc.date.accessioned2012-10-18T04:08:19Z
dc.date.available2012-10-18T04:08:19Z
dc.date.issued2012-07
dc.description.abstractBackground: Assessment of mitotic figures (MFs) is routinely practiced as prognostic indicator in oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC), but identification of MFs poses a problem in terms of staining characteristics. Aim: To evaluate effectiveness of crystal violet stain for staining of MFs and its comparison with hematoxylin and eosin (H and E) stain. Materials and Methods: Study sample includes archival tissues embedded in paraffin blocks diagnosed as OED (n = 30) and OSCC (n = 30). The control group comprised of tissue specimen from oral mucosa of healthy volunteers (n = 30). Two serial sections of each tissue specimen were stained separately with H and E stain and 1% crystal violet stain. The stained sections were observed under microscope for identification and counting of MFs. Data obtained was statistically analyzed by using the Man-Whitney U test. Results: A significant increase in number of MFs was observed in OED and OSCC in comparison with normal oral mucosa. There was a highly significant increase in number of MFs in crystal violet stained tissue sections when compared with H and E stain. Metaphase is the most commonly observed phase of mitosis in crystal violet stain when compared with H and E stain for all three groups. Conclusion: Crystal violet stain can be considered as selective stain for mitotic figures.en_US
dc.identifier.citationJadhav Kiran B, Mujib B R Ahmed, Gupta Nidhi. Crystal violet stain as a selective stain for the assessment of mitotic figures in oral epithelial dysplasia and oral squamous cell carcinoma. Indian Journal of Pathology & Microbiology. 2012 Jul-Sept 55(3): 283-287.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/142254
dc.language.isoenen_US
dc.source.urihttps://www.ijpmonline.org/article.asp?issn=0377-4929;year=2012;volume=55;issue=3;spage=283;epage=287;aulast=Jadhaven_US
dc.subjectCrystal violet stainen_US
dc.subjectH and E stainen_US
dc.subjectmitotic figuresen_US
dc.subjectoral epithelial dysplasiaen_US
dc.subjectoral squamous cell carcinomaen_US
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshCarcinoma, Squamous Cell --diagnosis
dc.subject.meshCarcinoma, Squamous Cell --pathology
dc.subject.meshFemale
dc.subject.meshGentian Violet --metabolism
dc.subject.meshHistocytochemistry --methods
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMicroscopy --methods
dc.subject.meshMitotic Index
dc.subject.meshMouth Mucosa --pathology
dc.subject.meshMouth Neoplasms --diagnosis
dc.subject.meshMouth Neoplasms --pathology
dc.subject.meshStaining and Labeling --methods
dc.subject.meshYoung Adult
dc.titleCrystal violet stain as a selective stain for the assessment of mitotic figures in oral epithelial dysplasia and oral squamous cell carcinoma.en_US
dc.typeArticleen_US
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