A modified technique to process peripheral nerves for light microscopy.

No Thumbnail Available
Date
1990-01-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A new technique is described to fix, paraffin embed and cut routine peripheral nerve biopsies for light microscopy. Buffered glutaraldehyde pH 7.4 is used as a common fixative for both light and electron microscopy. The nerve bits for LM are embedded in paraffin using the 'reverse embedding' method and blocks are trimmed to cut sections on the ultra microtome using glass knives. The uniformly thin sections can be utilised for any special stain and the good resolution of these thin sections facilitates better observation and documentation.
Description
Keywords
Citation
Rao TV, Ramamohan Y. A modified technique to process peripheral nerves for light microscopy. Indian Journal of Pathology & Microbiology. 1990 Jan; 33(1): 64-7
URI
https://imsear.searo.who.int/handle/123456789/74986
Collections
Indian Journal of Pathology & Microbiology