Role of immunophenotyping in characterisation of blast crisis of chronic myeloid leukemia--a study of 25 cases.
No Thumbnail Available
Date
1996-01-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The blast cell populations of 25 patients of chronic myeloid leukemia in blast crisis (CML-BC) were studied for morphological, cytochemical and immunophenotypic features. The patients were divided into 6 broad groups based upon the pattern of surface marker positivity-myeloblastic, mixed myeloblastic, megakaryoblastic, mixed lineage, lymphoid and undifferentiated blast crisis. Myeloperoxidase (MPO), Sudan Black B (SBB) and Chloroacetate esterase (CAE) stains showed 100% specificity for the myelomonocytic lineage but the sensitivity was low. Periodic acid Schiff (PAS) stain was neither specific nor sensitive for the lymphoid lineage. Immunophenotyping as compared to morphologic and cytochemical assessment, was seen to be most useful for assigning a lineage to leukemic cells in CML-BC.
Description
Keywords
Citation
Padmanabhan V, Varma N, Dash S, Varma S. Role of immunophenotyping in characterisation of blast crisis of chronic myeloid leukemia--a study of 25 cases. Indian Journal of Pathology & Microbiology. 1996 Jan; 39(1): 13-7