Characterization of mycobacterial antigens by Elisa and immunoblot methods.

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Date
1995-07-01
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Abstract
Antibodies to two mycobacterial antigens viz - culture filtrate antigen (CFA) and Mycobacterium tuberculosis antigen 5 were raised in rabbits. Enzyme-linked immuno sorbent assay (ELISA) and immunoblot methods were used for the evaluation of the specificity of the rabbit antibodies to M. tuberculosis. Immunoblot method is more sensitive than ELISA for the detection of antibodies to M. tuberculosis in the rabbit sera. It is being emphasised that characterisation of the mycobacterial antigens and evaluation of the specificity of the antimycobacterial antibodies are essential prior to their applications as an adjunct in the laboratory diagnosis of human mycobacterial disease.
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Mathai A, Radhakrishnan VV, Sehgal S. Characterization of mycobacterial antigens by Elisa and immunoblot methods. Indian Journal of Pathology & Microbiology. 1995 Jul; 38(3): 299-303
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https://imsear.searo.who.int/handle/123456789/73057
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Indian Journal of Pathology & Microbiology