Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies.
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1999-03-01
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Abstract
PURPOSE: In vitro study of the enzymes involved in aerobic, anaerobic and hexose monophosphate shunt in ultraviolet radiation exposed mice lenses.
METHOD: Of the selected enzymes, lactic dehydrogenase (LDH) was representative of anaerobic glucose oxidation, succinic dehydrogenase (SDH) of the aerobic oxidation, and Glucose-6-phosphate dehydrogenase (G-6-PDH) of the Hexose Monophosphate (HMP) shunt. Other enzymes studied were ATPase and glutathione reductase (GR).
RESULTS: Experiments with mice lenses in vitro showed that transparent lens became opaque following UV-irradiation at 360 nm. Opacification of the lens was accompanied by a change in enzyme activities for energy metabolism.
CONCLUSION: These changes were progressive in a manner analogous to sequential morphological changes, which would be crucial in maintaining lens transparency.
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Jain NK, Rawal UM. Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies. Indian Journal of Ophthalmology. 1999 Mar; 47(1): 25-9