Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles.
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2008-11-19
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Abstract
Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization.
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Hema M, Subba Reddy ChV, Savithri HS, Sreenivasulu P. Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles. Indian Journal of Experimental Biology. 2008 Nov; 46(11): 793-6