In vitro clonal propagation of Chlorophytum borivilianum Sant. et Fernand., a rare medicinal herb from immature floral buds along with inflorescence axis.

Sharma, Urvashi; Mohan, J S S

In vitro clonal propagation of Chlorophytum borivilianum Sant. et Fernand., a rare medicinal herb from immature floral buds along with inflorescence axis.

Date

2006-01-25

Authors

Sharma, Urvashi

Mohan, J S S

Abstract

A novel method of shoot regeneration from immature floral buds along with inflorescence axis in C. borivilianum, a rare medicinal herb is described. Using this explant, axenic cultures were established with very less contamination (10%). MS medium with 2 mg l(-1) kinetin and 0.1 mg l(-1) 2, 4-dichlorophenoxyacetic acid proved to be the best for multiple shoot induction. Maximum number (35) of shoot production was achieved in MS medium with 2 mg l(-1) benzylaminopurine. Rooting of shoots (86.7%) with maximum fasciculated roots (5) occurred on Knops medium containing iron and vitamins of MS medium with 2 mg l(-1) indole-3-butyric acid and 0.1% activated charcoal. Plant survival was 80% in four weeks after their removal from in vitro conditions. Per explant 34 hardened plants generated within 50 weeks. This protocol can be useful for large-scale clonal multiplication from immature floral buds with inflorescence axis and successfully used for germplasm conservation of this rare medicinal herb without destroying the mother plant.

Citation

Sharma U, Mohan JS. In vitro clonal propagation of Chlorophytum borivilianum Sant. et Fernand., a rare medicinal herb from immature floral buds along with inflorescence axis. Indian Journal of Experimental Biology. 2006 Jan; 44(1): 77-82