Immunoelectrophoresis of mycobacterial antigens.

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Date
1990-09-01
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Abstract
Polyvalent antiserum to culture filtrate of H37 Ra M. tuberculosis was raised in rabbits. Monospecific antiserum was raised against M. tuberculosis antigen-5, prepared from the culture filtrates by immunoabsorbent affinity chromatography. On immunoelectrophoresis, antigen-5 demonstrated single precipitin arc against polyvalent and monospecific antisera. The culture filtrate antigen demonstrated multiple precipitin arcs against polyvalent antiserum and single precipitin are against monospecific antiserum. Antigen-5 could be isolated and characterized from the culture filtrate of H37 Ra M. tuberculosis. Immunoelectrophoresis could be one of the method to characterize the mycobacterial antigens prepared in the laboratory.
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Radhakrishnan VV, Mathai A, Rao SB, Sehgal S. Immunoelectrophoresis of mycobacterial antigens. Indian Journal of Experimental Biology. 1990 Sep; 28(9): 812-5
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https://imsear.searo.who.int/handle/123456789/62741
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Indian Journal of Experimental Biology